Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / En "dual-addition" kalciumfluorescensanalys för…
JoVE Journal
Biochemistry

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

JoVE Journal Biochemistry
En "dual-addition" kalciumfluorescensanalys för screening med hög genomströmning av rekombinanta G-proteinkopplade receptorer
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
Journal (Biochemistry)
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
Journal (Biochemistry)
Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
 
Click here for the English version

En "dual-addition" kalciumfluorescensanalys för screening med hög genomströmning av rekombinanta G-proteinkopplade receptorer

Article DOI: 10.3791/64505-v • 08:46 min • December 2nd, 2022
December 2nd, 2022 •
1, 2, 1
1Department of Entomology, Texas A&M University, 2Department of Biochemistry and Biophysics, Texas A&M University

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

I detta arbete beskrivs en intracellulär kalciumfluorescensanalys med hög genomströmning för 384-brunnsplattor för att screena små molekylbibliotek på rekombinanta G-proteinkopplade receptorer (GPCR). Målet, kininreceptorn från nötkreatursfeberfästingen, Rhipicephalus microplus, uttrycks i CHO-K1-celler. Denna analys identifierar agonister och antagonister som använder samma celler i en "dual-addition" -analys.

Tags

Biokemi utgåva 190
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter