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Populatie- en eencellige analyse van antibioticapersistentie in Escherichia coli

Article DOI: 10.3791/64550-v • 12:29 min • March 24th, 2023
March 24th, 2023 •
*1, *1, 1, 1
1Bacterial Genetics and Physiology, Département de Biologie Moléculaire, Faculté des Sciences, Université Libre de Bruxelles (ULB)
* These authors contributed equally

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Antibioticapersistentie beschrijft het vermogen van kleine subpopulaties binnen een gevoelige isogene populatie om tijdelijk hoge doses bacteriedodende antibiotica te verdragen. Het huidige protocol combineert benaderingen om het fenotype van antibioticapersistentie op moleculair en cellulair niveau te karakteriseren na blootstelling van Escherichia coli aan dodelijke doses ofloxacine.

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Biologie Antibioticum persistentie populatieanalyse eencellige analyse Escherichia coli ofloxacine fluorescentiemicroscopie microfluïdica
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