Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Generation of Natural Killer Cells from Human…
JoVE Journal
Developmental Biology

이 콘텐츠은(는) 무료 액세스입니다.

JoVE Journal Developmental Biology
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
Determining Bile Duct Density in the Mouse Liver
Journal (Developmental Biology)
Determining Bile Duct Density in the Mouse Liver
Functional Cloning Using a Xenopus Oocyte Expression System
Journal (Developmental Biology)
Functional Cloning Using a Xenopus Oocyte Expression System
 

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Article DOI: 10.3791/64608-v • 06:53 min • January 13th, 2023
January 13th, 2023 •
1, 2, 2, 2,3, 2,3
1Major in Molecular Biology and Biotechnology, School of Biological Sciences, The University of Hong Kong, 2School of Biomedical Sciences, The University of Hong Kong, 3Centre for Translational Stem Cell Biology

챕터

요약
Automatic Translation

The present protocol shows how to differentiate CD3−/CD45+CD56+ cells with mild cytotoxicity from human expanded potential stem cells (hEPSCs) under both 3D and 2D culture conditions. This allows for routine phenotypical validation without the destruction of the complex microenvironment.

Tags

Natural Killer Cells Expanded Potential Stem Cells Cancer Immunotherapy HEPSCs DFK Medium Embryoid Bodies Trypsin Y-27632 Hemocytometer Petri Dish Incubate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter