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Chapters
- 00:05Introduction
- 00:51Pre-ELISA Steps: Prerequisites and Preprocessing
- 01:42ELISA Steps: Capture Antibody Coating and Blocking
- 02:28ELISA Steps: Application of Antigen and Serum Samples
- 03:16ELISA Steps: Enzyme-Conjugated Antibody Addition and Detection
- 04:02Results: Validation of Non-Adsorbed and Adsorbed Mycoplasma pneumoniae Antiserum
- 05:19Conclusion
In Mycoplasma pneumoniae infection, serology tests can generate good results, yet with low specificity because of immunological cross-reaction. The in-house antigen-capture ELISA, described in this paper, guarantees high species specificity and has been shown to be a reliable screening test for accurate diagnosis of M. pneumoniae.
Tags
Antigen-Capture ELISAMycoplasma PneumoniaeSpecific DetectionCross-reaction DepletionAdsorption TechniqueHigh SpecificityReliable Screening TestHeterologous Bacterial AntigensPolyclonal Anti-Mycoplasma Pneumoniae IgGCapture AntibodyBlocking SolutionMycoplasma Pneumoniae Whole ProteinsHuman Serum Test SamplesReference Positive And Negative Serum Controls
