Simple Continuous Glucose Monitoring in Freely Moving Mice

Doron Kleiman; Mika Littor; Mahmoud Nawas; Rachel Ben-Haroush Schyr; Danny Ben-Zvi

doi:10.3791/64743

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Simple Continuous Glucose Monitoring in Freely Moving Mice

자유롭게 움직이는 마우스의 간단한 연속 포도당 모니터링

Full Text

7,938 Views

03:25 min

February 24, 2023

DOI: 10.3791/64743-v

Doron Kleiman¹, Mika Littor^1,2, Mahmoud Nawas³, Rachel Ben-Haroush Schyr¹, Danny Ben-Zvi¹

¹Department of Developmental Biology and Cancer Research, Institute of Medical Research Israel-Canada,The Hebrew University-Hadassah Medical School, ²Department of Military Medicine and “Tzameret”, Faculty of Medicine,Hebrew University of Jerusalem, and Medical Corps, Israel Defense Forces, ³Department of Surgery,Hadassah Medical Center-Ein Kerem Campus

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study presents a straightforward method for implanting a commercial continuous glucose monitor (CGM) in mice to track glucose levels. The technique offers significant advantages, eliminating the need for complex microsurgery or bleeding, thus facilitating studies in obesity and diabetes models.

Key Study Components

Research Area

Diabetes research
Metabolic phenotype identification
Glucose monitoring in animal models

Background

Importance of monitoring glucose levels in obesity and diabetes research
Challenges associated with traditional surgical approaches
Need for effective methods to explore glucose management phenotypes

Methods Used

The procedure for inserting a CGM cannula
Mice as the biological model system
Utilization of suture techniques and sensor activation

Main Results

The method successfully secured the CGM cannula for extended monitoring
Data collected revealed new insights into metabolic phenotypes
Demonstrated feasibility and efficiency in data acquisition from mice

Conclusions

The study illustrates a reliable protocol for glucose monitoring in mice
This method contributes to advancing research in metabolic disorders

Frequently Asked Questions

What is the main advantage of this glucose monitoring method?

It simplifies the monitoring process and eliminates the need for invasive surgery or bleeding, making it less stressful for the mice.

How long can the CGM monitor glucose levels?

The method allows for continuous data collection, with effective monitoring over multiple days, as demonstrated in a three-day data collection period.

What biological models can benefit from this technique?

The technique is particularly useful in mouse models of obesity and diabetes, aiding in the identification of glucose management phenotypes.

What preparations are necessary before implanting the CGM?

It involves shaving the midline of the mouse's back, using depilatory cream, and making a small incision for cannula placement.

Are there any specific challenges when implementing this method?

The critical step is the careful insertion of the cannula and securing it properly to ensure it remains in place during the monitoring period.

Can the data collection parameters be adjusted?

Yes, the scripts provided allow modulation of various parameters during data analysis.

What type of data does this method produce?

The method produces continuous glucose monitoring data, which can reveal metabolic insights in mouse models.

여기에서는 환자를 위해 설계된 상업용 연속 포도당 모니터를 마우스에 이식하고 결과를 분석하기 위한 스크립트를 제공하는 간단한 방법을 설명합니다.

우리의 프로토콜은 생쥐의 포도당 수치를 모니터링하는 간단하고 저렴한 방법을 제공합니다. 이 기술의 가장 큰 장점은 까다로운 미세 수술이 필요하지 않으며 출혈이나 마우스 취급을 포함하지 않는다는 것입니다. 이 방법은 비만 및 당뇨병의 마우스 모델에서 파악하기 어려운 포도당 관리 표현형을 식별하는 데 사용할 수 있습니다.

이 프로토콜을 시도하는 동안 가장 중요한 단계는 캐뉼라를 피부 아래에 삽입하고 캐뉼라를 제자리에 유지하기 위해 내부 봉합사를 조이는 것입니다. 마취 된 마우스 뒤쪽의 정중선을 면도하기 시작하고 제모 크림을 사용하여 완전한 제모를 보장합니다. 센서를 열고 로드합니다.

View the full transcript and gain access to thousands of scientific videos