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A Silicosis Mouse Model Established by Repeated Inhalation of Crystalline Silica Dust

Article DOI: 10.3791/64862-v • 10:45 min • January 6th, 2023
January 6th, 2023 •
*1,2,3,4, *1,2,3,4, 1,3,4, 1,4, 1,4, 1,4, 1,2,3,4, 1,2,3,4
1Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, 2Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, 3Anhui Province Engineering Laboratory of Occupational Health and Safety, 4School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology
* These authors contributed equally

챕터

요약
Automatic Translation

This protocol describes a method for establishing a mouse model of silicosis through repeated exposure to silica suspensions via a nasal drip. This model can efficiently, conveniently, and flexibly mimic the pathological process of human silicosis with high repeatability and economy.

Tags

Medicine Mouse Model Inhalation Pathophysiology Screening Treatment Clinical Symptoms Acute/chronic Transformation Process Procedures CSD Powder Saline Suspension Ultrasonic Water Bath Anesthesia Aspiration Nostril Inhalation Mouse Cage
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