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TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

Article DOI: 10.3791/64953-v • 09:39 min • December 16th, 2022
December 16th, 2022 •
1,2, 1, 1, 1,3, 4, 1
1School of Neuroscience, Virginia Polytechnic Institute and State University, 2CMU-Pitt Joint Computational Biology, School of Medicine, University of Pittsburgh, 3Eastern Virginia Medical School, 4Department of Physics, University of Miami

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Summary
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TrackMate Analysis of Calcium Imaging (TACI) is an open-source ImageJ plugin for 3D calcium imaging analysis that examines motion on the z-axis and identifies the maximum value of each z-stack to represent a cell's intensity at the corresponding time point. It can separate neurons overlapping in the lateral (x/y) direction but on different z-planes.

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TACI ImageJ Plugin 3D Calcium Imaging Analysis X And Y Motion Correction Z Drift Correction User-friendly Open Source Motion Analysis Fly Larvae Preparation PBS Rinsing Glass Slide Thermocouple Micrprobe Glass Cover Slip Nail Polish Sealing Microscope Stage Focus Adjustment Laser Power Adjustment Z Stack Scanning Temperature Recording Fiji Installation Plugin Running
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