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Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence

Article DOI: 10.3791/65005-v • 08:07 min • April 28th, 2023
April 28th, 2023 •
1, 1, 2, 2, 1
1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL), Universidad de Buenos Aires, CONICET, 2Signalling Programme, The Babraham Institute

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The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification.

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Autophagy Levels LC3 Immunofluorescence Pancreatic Cell Models Agents Protein Aggregates Felipe Renna Malena Herrera Lopez Maria Ines Vaccaro Cell Preparation Cover Slips Ultraviolet Radiation DMEM FBS Penicillin Streptomycin Gemcitabine Solution Fixing And Permeabilizing Cells Methanol
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