Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

Marianna Gabrielle Almeida Galv&#227;o; Brisa Miranda Andrade Santos; Cah&#234; Moreira Aguiar; Adriana Bozzi

doi:10.3791/65722

Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididym...

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Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue(마우스 부고환 지방 조직에서 중간엽 줄기 세포의 분리, 체외 확장 및 특성 분석)

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04:53 min

January 12, 2024

DOI: 10.3791/65722-v

Marianna Gabrielle Almeida Galvão¹, Brisa Miranda Andrade Santos¹, Cahê Moreira Aguiar², Adriana Bozzi^1,2

¹Laboratory of Immunobiology, Department of Biological Sciences,State University of Santa Cruz - UESC, ²Department of Medicine,Afya Medical Sciences College

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Overview

This study focuses on the extraction, cultivation, and characterization of adipose tissue-derived stem cells (ADSCs) from the epididymal adipose tissue of Swiss mice. The findings highlight ADSCs' potential as a therapeutic tool, particularly in managing inflammation associated with diseases like Schistosoma mansoni infection.

Key Study Components

Research Area

Adipose-derived mesenchymal stem cells
Inflammation control
Therapeutic applications in infection

Background

High potential of adipose tissue as a source of stem cells
Represents an abundant and accessible source
Defines a protocol for meticulous collection and processing

Methods Used

Isolation and culture of ADSCs
Swiss mice as the biological model
Characterization techniques including staining and morphological assessment

Main Results

Demonstrated ADSCs' multi-lineage differentiation potential
Effective control of granulomatous reactions in Schistosoma mansoni infection
Characterization confirmed morphology and potential of ADSCs

Conclusions

This study provides a reliable protocol for ADSC extraction and characterization
Highlights the therapeutic promise of ADSCs in inflammatory diseases

Frequently Asked Questions

What are adipose-derived stem cells?

Adipose-derived stem cells (ADSCs) are multipotent stem cells found in adipose tissue, capable of differentiating into various cell types.

How were the stem cells extracted in this study?

Stem cells were extracted from the epididymal adipose tissue of Swiss mice following aseptic techniques and specific processing methods.

What diseases could benefit from ADSC therapy?

Potential applications include treatment for inflammatory diseases and tissue regeneration.

What is the significance of this research?

This research fills a gap in the techniques for isolating ADSCs and demonstrates their therapeutic potential in a disease model.

What methodologies were used for characterization?

Techniques included morphological assessments and staining methods to evaluate differentiation potential.

Can ADSCs be used in combination with other therapies?

Yes, the study indicates that ADSCs can be combined with other treatments, such as praziquantel, to improve therapeutic outcomes.

지방 조직은 중간엽 줄기 세포의 훌륭한 공급원입니다. 여기에서는 스위스 생쥐 부고환 지방 조직에서 지방 조직 유래 줄기 세포(ADSC)의 단계별 추출, 배양 및 특성 분석을 제공합니다.

제 연구는 여러 질병의 염증을 개선하기 위해 다른 치료법과 연관되거나 연관되지 않은 새로운 접근법 치료법으로 지방 유래 중간엽 줄기 세포에 중점을 둡니다. 우리는 지방 중간엽 줄기 세포를 프라지콴텔과 함께 사용하여 주혈흡충 만소니 감염에서 육아종 반응을 크게 제어했음을 입증하여 이 결합 치료 접근법의 이점을 강조했습니다. 본 프로토콜은 무균 질환 하에서 부고환 지방 조직을 꼼꼼하게 채취하는 과정에서 발생하는 물리적 처리와 격렬한 흔들림을 수반하는 연구 격차를 해결하고, 지방 유래 줄기세포를 활용하기 위한 최적의 시기를 식별합니다.

당사의 프로토콜은 상당한 이점을 제공하며, 특히 중간엽 줄기 세포의 분리를 위한 풍부한 가용성과 잠재적 강도를 제공합니다. 또한 중간엽 줄기 세포는 항염증 및 조직 재생 능력을 포함한 강력한 치료 특성을 나타냅니다.

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