A Passive Ankle Dorsiflexion Testing System for an <em>In Vivo</em> Model of Overuse-induced Tendinopathy

Pooja H. Chainani; Patrick M. Williamson; Diana Yeritsyan; Kaveh Momenzadeh; Nadim Kheir; Joseph P. DeAngelis; Arun J. Ramappa; Ara Nazarian

doi:10.3791/65803

과사용 유발 건병증의 In Vivo 모델을 위한 수동 발목 배굴곡 검사 시스템

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

A Passive Ankle Dorsiflexion Testing System for an In Vivo Model of Overuse-induced Tendinopathy

과사용 유발 건병증의 In Vivo 모델을 위한 수동 발목 배굴곡 검사 시스템

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04:37 min

March 1, 2024

DOI: 10.3791/65803-v

Pooja H. Chainani^1,2, Patrick M. Williamson^1,2, Diana Yeritsyan¹, Kaveh Momenzadeh¹, Nadim Kheir¹, Joseph P. DeAngelis^1,3, Arun J. Ramappa^1,3, Ara Nazarian^1,2,3,4

¹Musculoskeletal Translational Innovation Initiative, Carl J. Shapiro Department of Orthopaedic Surgery,Beth Israel Deaconess Medical Center, Harvard Medical School, ²Mechanical Engineering Department,Boston University, ³Carl J. Shapiro Department of Orthopaedic Surgery,Beth Israel Deaconess Medical Center, Harvard Medical School, ⁴Department of Orthopaedic Surgery,Yerevan State Medical University

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Overview

This protocol presents a testing system used to induce quantifiable and controlled fatigue injuries in a rat Achilles tendon for an in-vivo model of overuse-induced tendinopathy. The model allows for continuous measurements of tendon stresses and strains during injuries and the associated biological changes.

Key Study Components

Area of Science

Neuroscience
Biology
Tendinopathy Research

Background

Overuse-induced tendinopathy is a common injury in various populations.
Current evaluation tools are limited to end-of-stage tissue harvesting.
In vivo models provide physiologically relevant loading of tendons.
Ex vivo models allow measurement of biological responses to injuries.

Purpose of Study

To investigate mechano-biological factors affecting tendon injury response.
To assess the impact of exercise frequency, magnitude, and duration on tendons.
To combine in vivo and ex vivo measures for comprehensive analysis.

Methods Used

Securing rat's ankle to a joint actuator for passive dorsiflexion.
Utilizing a custom MATLAB script for controlled testing.
Employing ultrasound and MRI for continuous measurement.
Combining mechanical measures, histology, and biological assays.

Main Results

Direct measurement of tendon stresses and strains during injuries.
Insights into biological changes associated with overuse injuries.
Enhanced understanding of factors influencing tendon injury response.
Potential for improved evaluation of tendinopathy treatments.

Conclusions

The developed model allows for real-time assessment of tendon injuries.
Combining in vivo and ex vivo approaches offers a comprehensive view.
Findings may inform future research and clinical practices in tendinopathy.

Frequently Asked Questions

What is the significance of this study?

This study provides a novel in vivo model to better understand overuse-induced tendinopathy and its biological implications.

How does the testing system work?

The system secures the rat's ankle and uses a joint actuator for controlled passive dorsiflexion to induce fatigue injuries.

What measurements can be taken with this model?

The model allows for continuous measurements of tendon stresses, strains, and associated biological changes using ultrasound and MRI.

What are the limitations of current evaluation tools?

Current tools are limited to end-of-stage tissue harvesting, which does not allow for real-time assessment of tendon responses.

What factors are being investigated in this study?

The study investigates the frequency, magnitude, and duration of exercise loads on tendon injury responses.

How can this research impact clinical practices?

The findings may lead to improved evaluation and treatment strategies for tendinopathy in clinical settings.

이 프로토콜은 남용 유발 건병증의 생체 내 모델에 대해 쥐 아킬레스건에서 정량화 가능하고 통제된 피로 부상을 유도하는 데 사용되는 테스트 시스템을 제시합니다. 이 절차는 쥐의 발목을 관절 액추에이터에 고정하는 것으로 구성되며, 이 액추에이터는 맞춤형으로 작성된 MATLAB 스크립트로 수동 발목 배측굴곡을 수행합니다.

우리의 연구는 과사용 유도 건병증의 시작과 진행에 관여하는 기계적 생물학적 요인을 다룹니다. 우리가 대답하려는 질문에는 부상에 대한 힘줄의 반응에 영향을 미치는 빈도, 크기 및 지속 시간과 같은 운동의 요인이 포함됩니다. 결과 측정을 평가하기 위한 기계적 측정, 조직학 및 생물학적 분석과 같은 현재 도구는 중앙 집중화되어 있으며 최종 단계 조직 채취로 제한됩니다.

이 모델을 사용하면 초음파 및 MRI와 같은 도구를 사용하여 생체 내 연속 측정을 탐색할 수 있습니다 현장에서 생체 내 모델은 힘줄과 생리학적으로 관련된 매너를 로드하는 반면 생체 외 모델은 부상에 대한 생물학적 반응을 측정할 수 있습니다. 우리의 프로토콜은 부상 및 관련 생물학적 변화 중 힘줄 응력과 긴장을 직접 측정하기 위해 이 두 가지 측정을 결합합니다. 힘줄 부상을 유발하는 적응 시스템을 구축하여 기계적 요인과 생물학적 요인을 동시에 탐색할 수 있습니다.

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