Neurotoxicity Assessment in Adult <em>Danio rerio</em> using a Battery of Behavioral Tests in a Single Tank

Juliette Bedrossiantz; Eva Prats; Demetrio Rald&#250;a

doi:10.3791/65869

단일 탱크에서 행동 테스트 배터리를 사용한 성인 Danio rerio 의 신경 독성 평가

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Neurotoxicity Assessment in Adult Danio rerio using a Battery of Behavioral Tests in a Single Tank

단일 탱크에서 행동 테스트 배터리를 사용한 성인 Danio rerio 의 신경 독성 평가

Full Text

2,322 Views

08:36 min

November 3, 2023

DOI: 10.3791/65869-v

Juliette Bedrossiantz¹, Eva Prats², Demetrio Raldúa¹

¹Institute for Environmental Assessment and Water Research (IDAEA-CSIC), ²Research and Development Center (CID-CSIC)

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study introduces a behavioral test battery, including the novel tank, shoaling, and social preference tests, to investigate the neurotoxic effects of chemicals like methamphetamine and glyphosate on adult zebrafish. The protocol emphasizes reproducibility and sensitivity to genetic and pharmacological manipulations, enhancing the understanding of environmental neurotoxicity.

Key Study Components

Area of Science

Neurotoxicity
Behavioral pharmacology
Environmental research

Background

Investigates behavioral effects of pollutants and drugs in adult zebrafish.
Aims to understand underlying mechanisms of these behaviors.
Focuses on asocial and anxiety-like behaviors that cannot be assessed in embryos.
Established assays are sensitive and facilitate collaborative research.

Purpose of Study

To assess behavioral effects of neurotoxic chemicals on adult zebrafish.
To standardize behavioral tests for better reproducibility.
To explore mechanisms leading to observed behavioral changes.

Methods Used

Utilizes a behavioral test battery in a single tank setup for zebrafish.
Focuses on adult zebrafish as the primary biological model.
No multiomics or metabolic analyses were mentioned.
Involves configuring experimental tanks and using video analysis for behavioral assessment.
Outlines detailed steps for aquaria setup, video recording, and data analysis.

Main Results

Behavioral tests indicated significant changes in zebrafish exposed to methamphetamine, such as decreased exploration and increased anxiety.
Social behavior tests showed isolation phenotypes with increased distances between individuals after exposure.
Identified behavioral adjustments, enhancing understanding of neurotoxic impacts.

Conclusions

The study validates a comprehensive method for assessing neurotoxic impacts in zebrafish.
Enhances knowledge of environmental pollutants' effects on behavior.
Supports advancement in understanding neurotoxicity mechanisms relevant to environmental and pharmacological research.

Frequently Asked Questions

What are the advantages of using zebrafish in this research?

Zebrafish are transparent at early stages, allowing for non-invasive observation of behavior and development. They also have well-understood genetics and rapid development, making them ideal for behavioral studies.

How is the behavioral testing implemented?

Behavioral tests are conducted in specially configured tanks, where fish are recorded during specific assays such as the novel tank test or social preference test.

What types of data are collected during the experiments?

Data includes measures of distance traveled, time spent in various zones, and social interactions, providing insight into the anxiety and social behaviors of zebrafish.

How can these methods be adapted for other studies?

The protocols can be modified for different environmental pollutants or behavioral assays, ensuring flexibility for various research questions.

What limitations should be considered when using this model?

While zebrafish provide a valuable model, differences in brain structure and function compared to mammals must be considered when extrapolating results to other species.

How does this research contribute to understanding neurotoxicity?

By systematically assessing behavioral changes, this research helps elucidate the effects of environmental chemicals on neural mechanisms and behavior.

Why are behavioral assays important in neurotoxicology?

Behavioral assays offer insights into the functional impacts of neurotoxins, bridging molecular and organism-level effects, and revealing potential underlying mechanisms of toxicity.

여기에서는 단일 수조를 사용하여 성체 제브라피쉬에 대한 화학 물질(예: 메스암페타민 및 글리포세이트)의 잠재적인 신경 독성 영향을 효과적으로 결정하기 위해 새로운 수조, Shoaling 및 사회적 선호도 테스트를 포함한 포괄적인 행동 테스트 배터리를 제시합니다. 이 방법은 신경 독성 및 환경 연구와 관련이 있습니다.

우리의 주요 연구는 어류의 환경 오염 물질과 약물에 의해 유발되는 행동 영향을 식별하는 것을 목표로 합니다. 또한 이러한 효과로 이어지는 메커니즘을 이해하려고 노력합니다. 사회적, 불안적 행동은 배아를 사용하여 편리하게 해결할 수 없습니다.

우리는 성인 제브라피시 모델에서 이러한 행동을 목표로 하는 연구를 수행합니다. 이 프로토콜은 테스트의 재현성을 촉진하고 과학계 내에서 얻은 결과를 조화시키기 위해 기본 재료 자원으로 행동 도구를 구현하는 방법을 자세히 설명합니다. 이 프로토콜에 제시된 분석은 재현성이 높고 유전 및 약리학적 조작에 민감한 것으로 입증되어 행동의 기본 신경과 분자 메커니즘을 촉진하는 가변적이고 비침습적인 도구입니다.

일단 행동적 노력이 확인되면, 가장 어려운 부분은 관찰적 노력으로 이어지는 구체적인 행동 방식을 결정하는 것이다. 예측이 분자 개시 사건에 대한 몇 가지 단서를 제공할 수 있는 분자 개시 사건부터 시작하여, 정확한 예측을 얻기까지는 아직 멀었습니다. 우리는 현재 MPTP 및 로테논을 사용하여 배아 및 성인 제브라피시에서 개발된 파킨슨병의 화학적 모델의 위상 타당도를 결정하는 데 관심이 있으며, 보행과 같은 움직임의 운동학 또는 음향 유발 탈출 반응과 같은 폭발적인 움직임 중에 평가될 것입니다.

View the full transcript and gain access to thousands of scientific videos