Chronic Constriction Injury of the Distal Infraorbital Nerve (DIoN-CCI) in Mice to Study Trigeminal Neuropathic Pain

Kristof R. Deseure; Guy H. Hans

doi:10.3791/66420

삼차신경병증성 통증을 연구하기 위한 마우스의 원위 안와하부 신경(DIoN-CCI)의 만성 수축 손상

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Neuroscience
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JoVE Journal Neuroscience

Chronic Constriction Injury of the Distal Infraorbital Nerve (DIoN-CCI) in Mice to Study Trigeminal Neuropathic Pain

삼차신경병증성 통증을 연구하기 위한 마우스의 원위 안와하부 신경(DIoN-CCI)의 만성 수축 손상

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05:38 min

March 8, 2024

DOI: 10.3791/66420-v

Kristof R. Deseure^1,2, Guy H. Hans^1,2

¹Antwerp Surgical Training, Anatomy and Research Centre, Algology (pain research),University of Antwerp, ²Multidisciplinary Pain Center,Antwerp University Hospital

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Overview

This study investigates the effects of chronic constriction injury of the distal infraorbital nerve in mice as a model for trigeminal neuropathic pain. The model allows researchers to explore spontaneous behavioral changes and nocifensive responses, highlighting the nuances of ongoing pain and allodynia.

Key Study Components

Area of Science

Neuroscience
Neuropathic Pain Models
Behavioral Analysis in Mice

Background

Trigeminal neuropathic pain is a significant clinical issue that affects the quality of life.
Comparative studies typically focus on sensory reflex-based pain testing, which often overlooks ongoing pain behaviors.
The distal infraorbital nerve approach is less invasive and has the potential for broader applicability in pain research.
Understanding spontaneous neuropathic pain responses is critical for developing effective treatment strategies.

Purpose of Study

To perform an infraorbital nerve injury to study the development of trigeminal neuropathic pain.
To broaden the understanding of spontaneous and ongoing pain in animal models.
To explore potential new treatment strategies for neuropathic pain conditions.

Methods Used

Involves a surgical model where the distal infraorbital nerve is ligated in mice.
The study assesses spontaneous face grooming behaviors and responses to von Frey hair stimulation following nerve injury.
Observations are conducted over a timeline of six weeks post-injury to capture changes in behavior.
Behavioral recordings are analyzed to quantify face grooming episodes and mechanical responsiveness.

Main Results

Mice exhibited increased face grooming activity and altered responses to tactile stimulation indicative of ongoing pain and allodynia.
Significant postoperative changes in grooming behaviors peaked in the first week but remained elevated for six weeks.
Initially, there was unresponsiveness to mechanical stimulation, transitioning into hyperresponsiveness over time.

Conclusions

This study provides insights into the behavioral manifestations of trigeminal neuropathic pain in mice.
It emphasizes the importance of assessing spontaneous pain behaviors in understanding neuropathic pain.
The findings could aid in the development of novel treatments for trigeminal pain conditions.

Frequently Asked Questions

What are the advantages of using the distal infraorbital nerve model?

The distal approach is less invasive, requiring simplified surgical techniques that can be performed without a stereotaxic frame, making it more accessible for researchers.

How is the infraorbital nerve injury implemented in mice?

The procedure involves a skin incision to expose the infraorbital nerve, followed by ligation to induce injury while minimizing damage to surrounding tissues.

What types of behavioral data are measured?

The study evaluates spontaneous behaviors, specifically face grooming episodes, and responses to mechanical stimulation using von Frey hairs.

How can the findings be applied in clinical contexts?

Insights from this model may lead to improved understanding and treatment options for human trigeminal neuropathic pain, enhancing patient quality of life.

What limitations should be considered when interpreting the results?

While the model offers valuable insights, the complexity of human pain conditions and individual variability should be considered when translating findings to clinical settings.

생쥐에서 원위 안와하부 신경의 만성 수축 손상은 촉각 자극에 대한 반응으로 자발적 행동(얼굴 손질 활동 증가)과 통각 행동(von Frey 모발 자극에 대한 과민성)의 변화를 유발하며, 이는 지속적인 통증 및 이질증의 징후이며 삼차신경병증성 통증의 모델 역할을 합니다.

이 절차의 전반적인 목표는 생쥐에서 안와하부 신경 손상을 수행하여 삼차신경병증성 통증의 발달을 연구하고 새로운 치료 전략을 테스트하는 것입니다. 안와하 신경 결찰 후 고립된 단계 그루밍 행동은 자발적 신경병증성 통증을 연구하는 몇 안 되는 동물 모델 중 하나입니다. 그러나 그 사용은 안와하 신경 결찰술을 수행하는 복잡성으로 인해 오랫동안 방해를 받아 왔습니다.

열이나 추위 자극으로 인한 몸싸움이나 금단 반응과 같은 감각 반사 기반 통증 검사와 비교할 때, 자발적이거나 지속적인 통증을 측정하는 것은 삶의 질에 영향을 미치는 중요한 임상 증상이라는 점을 고려할 때 충분한 관심을 받지 못했습니다. 안와하부 신경에 대한 원위 접근법은 안와 내 접근법보다 외과적으로 덜 까다로운 절차라는 장점이 있습니다. 이 검사는 최소한으로 침습적이며, 입체적 프레임을 사용하지 않고 수행할 수 있습니다.

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