Name: 간세포암(Hepatocellular Carcinoma)의 전신 및 국소 면역 반응에 대한 질량 세포 분석 (동영상) | JoVE Journal
Uploaded: 2025-04-25T13:00:00.000+00:00
Duration: 8 min 25 s
Description: 학습하세요 간세포암의 전신 및 국소 면역 반응을 질량 세포 분석(CyTOF)으로 평가하는 방법을. 단일 세포 수준에서 면역 세포를 프로파일링하여 14개의 독특한 세포 유형을 식별하고 종양 진행과 관련된 면역 회피 메커니즘을 이해할 수 있습니다.

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5 minutes

<ol><li>Zhang, C. H., Cheng, Y., Zhang, S., Fan, J., Gao, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Changing+epidemiology+of+hepatocellular+carcinoma+in+Asia%5BTitle%5D)+AND+%22Liver+Int%22%5BJournal%5D)">Changing epidemiology of hepatocellular carcinoma in Asia.</a> Liver Int. 42 (9), 2029-2041 (2022).</li>
<li>Renne, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hepatocellular+carcinoma%3A+A+clinical+and+pathological+overview%5BTitle%5D)+AND+%22Pathologica%22%5BJournal%5D)">Hepatocellular carcinoma: A clinical and pathological overview.</a> Pathologica. 113 (3), 203-217 (2021).</li>
<li>Sayiner, M., Golabi, P., Younossi, Z. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Disease+burden+of+hepatocellular+carcinoma%3A+A+global+perspective%5BTitle%5D)+AND+%22Dig+Dis+Sci%22%5BJournal%5D)">Disease burden of hepatocellular carcinoma: A global perspective.</a> Dig Dis Sci. 64 (4), 910-917 (2019).</li>
<li>Zhang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Risk+factors+and+prevention+of+viral+hepatitis-related+hepatocellular+carcinoma%5BTitle%5D)+AND+%22Front+Oncol%22%5BJournal%5D)">Risk factors and prevention of viral hepatitis-related hepatocellular carcinoma.</a> Front Oncol. 11, 686962(2021).</li>
<li>Zou, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Economic+burden+and+quality+of+life+of+hepatocellular+carcinoma+in+greater+China%3A+A+systematic+review%5BTitle%5D)+AND+%22Front+Public+Health%22%5BJournal%5D)">Economic burden and quality of life of hepatocellular carcinoma in greater China: A systematic review.</a> Front Public Health. 10, 801981(2022).</li>
<li>Li, Y., You, Z., Tang, R., Ma, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tissue-resident+memory+t+cells+in+chronic+liver+diseases%3A+Phenotype%2C+development+and+function%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Tissue-resident memory t cells in chronic liver diseases: Phenotype, development and function.</a> Front Immunol. 13, 967055(2022).</li>
<li>Shen, K. Y., Zhu, Y., Xie, S. Z., Qin, L. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunosuppressive+tumor+microenvironment+and+immunotherapy+of+hepatocellular+carcinoma%3A+Current+status+and+prospectives%5BTitle%5D)+AND+%22J+Hematol+Oncol%22%5BJournal%5D)">Immunosuppressive tumor microenvironment and immunotherapy of hepatocellular carcinoma: Current status and prospectives.</a> J Hematol Oncol. 17 (1), 25(2024).</li>
<li>Oura, K., Morishita, A., Tani, J., Masaki, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tumor+immune+microenvironment+and+immunosuppressive+therapy+in+hepatocellular+carcinoma%3A+A+review%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Tumor immune microenvironment and immunosuppressive therapy in hepatocellular carcinoma: A review.</a> Int J Mol Sci. 22 (11), 5801(2021).</li>
<li>Chen, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+infiltrating+immune+cells+in+tumor+microenvironment+on+metastasis+of+hepatocellular+carcinoma%5BTitle%5D)+AND+%22Cell+Oncol%22%5BJournal%5D)">Effect of infiltrating immune cells in tumor microenvironment on metastasis of hepatocellular carcinoma.</a> Cell Oncol. 46 (6), 1595-1604 (2023).</li>
<li>Du, Q., An, Q., Zhang, J., Liu, C., Hu, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Unravelling+immune+microenvironment+features+underlying+tumor+progression+in+the+single-cell+era%5BTitle%5D)+AND+%22Cancer+Cell+Int%22%5BJournal%5D)">Unravelling immune microenvironment features underlying tumor progression in the single-cell era.</a> Cancer Cell Int. 24 (1), 143(2024).</li>
<li>Xu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Reshaping+the+systemic+tumor+immune+environment+%28stie%29+and+tumor+immune+microenvironment+%28time%29+to+enhance+immunotherapy+efficacy+in+solid+tumors%5BTitle%5D)+AND+%22J+Hematol+Oncol%22%5BJournal%5D)">Reshaping the systemic tumor immune environment (stie) and tumor immune microenvironment (time) to enhance immunotherapy efficacy in solid tumors.</a> J Hematol Oncol. 15 (1), 87(2022).</li>
<li>Mao, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Crosstalk+between+cancer-associated+fibroblasts+and+immune+cells+in+the+tumor+microenvironment%3A+New+findings+and+future+perspectives%5BTitle%5D)+AND+%22Mol+Cancer%22%5BJournal%5D)">Crosstalk between cancer-associated fibroblasts and immune cells in the tumor microenvironment: New findings and future perspectives.</a> Mol Cancer. 20 (1), 131(2021).</li>
<li>Roederer, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spectral+compensation+for+flow+cytometry%3A+Visualization+artifacts%2C+limitations%2C+and+caveats%5BTitle%5D)+AND+%22Cytometry%22%5BJournal%5D)">Spectral compensation for flow cytometry: Visualization artifacts, limitations, and caveats.</a> Cytometry. 45 (3), 194-205 (2001).</li>
<li>Sahaf, B., Rahman, A., Maecker, H. T., Bendall, S. C. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSahaf%2C+B+%22Biomarkers+for+immunotherapy+of+cancer%3A+Methods+and+protocols%22">Biomarkers for immunotherapy of cancer: Methods and protocols</a>. , Springer. New York, New York, NY. (2020).</li>
<li>Turaç, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Combined+flow+cytometric+analysis+of+surface+and+intracellular+antigens+reveals+surface+molecule+markers+of+human+neuropoiesis%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Combined flow cytometric analysis of surface and intracellular antigens reveals surface molecule markers of human neuropoiesis.</a> PLoS One. 8 (6), e68519(2013).</li>
<li>Wu, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Redefining+tumor-associated+macrophage+subpopulations+and+functions+in+the+tumor+microenvironment%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Redefining tumor-associated macrophage subpopulations and functions in the tumor microenvironment.</a> Front Immunol. 11, 1731(2020).</li>
<li>Elaldi, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High+dimensional+imaging+mass+cytometry+panel+to+visualize+the+tumor+immune+microenvironment+contexture%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">High dimensional imaging mass cytometry panel to visualize the tumor immune microenvironment contexture.</a> Front Immunol. 12, 666233(2021).</li>
<li>Gadalla, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Validation+of+cytof+against+flow+cytometry+for+immunological+studies+and+monitoring+of+human+cancer+clinical+trials%5BTitle%5D)+AND+%22Front+Oncol%22%5BJournal%5D)">Validation of cytof against flow cytometry for immunological studies and monitoring of human cancer clinical trials.</a> Front Oncol. 9, 415(2019).</li>
<li>Ijsselsteijn, M. E., Van Der Breggen, R., Farina Sarasqueta, A., Koning, F., De Miranda, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+40-marker+panel+for+high+dimensional+characterization+of+cancer+immune+microenvironments+by+imaging+mass+cytometry%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">A 40-marker panel for high dimensional characterization of cancer immune microenvironments by imaging mass cytometry.</a> Front Immunol. 10, 2534(2019).</li>
<li>Zhang, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mass+cytometry-based+peripheral+blood+analysis+as+a+novel+tool+for+early+detection+of+solid+tumours%3A+A+multicentre+study%5BTitle%5D)+AND+%22Gut%22%5BJournal%5D)">Mass cytometry-based peripheral blood analysis as a novel tool for early detection of solid tumours: A multicentre study.</a> Gut. 72 (5), 996-1006 (2023).</li>
<li>Zheng, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Trajectory+and+functional+analysis+of+pd-1%28high%29+cd4%28%2B%29cd8%28%2B%29+t+cells+in+hepatocellular+carcinoma+by+single-cell+cytometry+and+transcriptome+sequencing%5BTitle%5D)+AND+%22Adv+Sci%22%5BJournal%5D)">Trajectory and functional analysis of pd-1(high) cd4(+)cd8(+) t cells in hepatocellular carcinoma by single-cell cytometry and transcriptome sequencing.</a> Adv Sci. 7 (13), 2000224(2020).</li>
<li>Song, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protocol+for+isolating+single+cells+from+human+pancreatic+cancer+tissues+and+analyzing+major+immune+cell+populations+using+flow+cytometry%5BTitle%5D)+AND+%22STAR+Protoc%22%5BJournal%5D)">Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry.</a> STAR Protoc. 4 (4), 102679(2023).</li>
<li>Simoni, Y., Chng, M. H. Y., Li, S., Fehlings, M., Newell, E. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mass+cytometry%3A+A+powerful+tool+for+dissecting+the+immune+landscape%5BTitle%5D)+AND+%22Curr+Opin+Immunol%22%5BJournal%5D)">Mass cytometry: A powerful tool for dissecting the immune landscape.</a> Curr Opin Immunol. 51, 187-196 (2018).</li>
<li>Comi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coexpression+of+cd163+and+cd141+identifies+human+circulating+il-10-producing+dendritic+cells+%28dc-10%29%5BTitle%5D)+AND+%22Cell+Mol+Immunol%22%5BJournal%5D)">Coexpression of cd163 and cd141 identifies human circulating il-10-producing dendritic cells (dc-10).</a> Cell Mol Immunol. 17 (1), 95-107 (2020).</li>
<li>Fan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-dimensional+single-cell+analysis+delineates+peripheral+immune+signature+of+coronary+atherosclerosis+in+human+blood%5BTitle%5D)+AND+%22Theranostics%22%5BJournal%5D)">High-dimensional single-cell analysis delineates peripheral immune signature of coronary atherosclerosis in human blood.</a> Theranostics. 12 (15), 6809-6825 (2022).</li>
<li>Sheng, J., et al. Human endogenous retrovirus activation contributes to biliary atresia pathogenesis through re-education of resident macrophages. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSheng%2C+J+%22Biorxiv%22">Biorxiv</a>. , (2022).</li>
<li>Terekhova, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+atlas+of+healthy+human+blood+unveils+age-related+loss+of+nkg2c%28%2B%29gzmb%28-%29cd8%28%2B%29+memory+t+cells+and+accumulation+of+type+2+memory+t+cells%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Single-cell atlas of healthy human blood unveils age-related loss of nkg2c(+)gzmb(-)cd8(+) memory t cells and accumulation of type 2 memory t cells.</a> Immunity. 56 (12), 2836-2854.e9 (2023).</li>
<li>Sathaliyawala, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Distribution+and+compartmentalization+of+human+circulating+and+tissue-resident+memory+t+cell+subsets%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Distribution and compartmentalization of human circulating and tissue-resident memory t cell subsets.</a> Immunity. 38 (1), 187-197 (2013).</li>
<li>Lee, S., Vu, H. M., Lee, J. H., Lim, H., Kim, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advances+in+mass+spectrometry-based+single+cell+analysis%5BTitle%5D)+AND+%22Biology%22%5BJournal%5D)">Advances in mass spectrometry-based single cell analysis.</a> Biology. 12 (3), 395(2023).</li>
<li>Iyer, A., Hamers, A. A. J., Pillai, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cytof+for+the+masses%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Cytof for the masses.</a> Front Immunol. 13, 815828(2022).</li>
<li>Yuan, X., Wang, J., Huang, Y., Shangguan, D., Zhang, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+profiling+to+explore+immunological+heterogeneity+of+tumor+microenvironment+in+breast+cancer%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Single-cell profiling to explore immunological heterogeneity of tumor microenvironment in breast cancer.</a> Front Immunol. 12, 643692(2021).</li>
<li>Serban, G. M., Mănescu, I. B., Manu, D. R., Dobreanu, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+a+density+gradient+centrifugation+protocol+for+isolation+of+peripheral+blood+mononuclear+cells%5BTitle%5D)+AND+%22Acta+Marisiensis+-+Seria+Medica%22%5BJournal%5D)">Optimization of a density gradient centrifugation protocol for isolation of peripheral blood mononuclear cells.</a> Acta Marisiensis - Seria Medica. 64 (2), 83-90 (2018).</li>
<li>Shcherbakova, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Factors+affecting+cell+viability+during+the+enzymatic+dissociation+of+human+endocrine+tumor+tissues%5BTitle%5D)+AND+%22Biology+%28Basel%29%22%5BJournal%5D)">Factors affecting cell viability during the enzymatic dissociation of human endocrine tumor tissues.</a> Biology (Basel). 13 (9), 665(2024).</li>
<li>Devine, R. D., Alkhalaileh, H. S., Lyberger, J. M., Behbehani, G. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Alternative+methods+of+viability+determination+in+single+cell+mass+cytometry%5BTitle%5D)+AND+%22Cytometry+A%22%5BJournal%5D)">Alternative methods of viability determination in single cell mass cytometry.</a> Cytometry A. 99 (10), 1042-1053 (2021).</li>
<li>Gonzalez, V. D., Huang, Y. W., Fantl, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mass+cytometry+for+the+characterization+of+individual+cell+types+in+ovarian+solid+tumors%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Mass cytometry for the characterization of individual cell types in ovarian solid tumors.</a> Methods Mol Biol. 2424, 59-94 (2022).</li>
<li>Zabransky, D. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Profiling+of+syngeneic+mouse+hcc+tumor+models+as+a+framework+to+understand+anti-pd-1+sensitive+tumor+microenvironments%5BTitle%5D)+AND+%22Hepatology%22%5BJournal%5D)">Profiling of syngeneic mouse hcc tumor models as a framework to understand anti-pd-1 sensitive tumor microenvironments.</a> Hepatology. 77 (5), 1566-1579 (2023).</li>
<li>Levine, L. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+analysis+by+mass+cytometry+reveals+metabolic+states+of+early-activated+cd8%28%2B%29+t+cells+during+the+primary+immune+response%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Single-cell analysis by mass cytometry reveals metabolic states of early-activated cd8(+) t cells during the primary immune response.</a> Immunity. 54 (4), 829-844.e5 (2021).</li>
<li>Lv, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunotherapy%3A+Reshape+the+tumor+immune+microenvironment%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Immunotherapy: Reshape the tumor immune microenvironment.</a> Front Immunol. 13, 844142(2022).</li>
<li>Zhou, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deciphering+the+tumor+immune+microenvironment+from+a+multidimensional+omics+perspective%3A+Insight+into+next-generation+car-t+cell+immunotherapy+and+beyond%5BTitle%5D)+AND+%22Mol+Cancer%22%5BJournal%5D)">Deciphering the tumor immune microenvironment from a multidimensional omics perspective: Insight into next-generation car-t cell immunotherapy and beyond.</a> Mol Cancer. 23 (1), 131(2024).</li>
<li>Hsieh, W. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spatial+multi-omics+analyses+of+the+tumor+immune+microenvironment%5BTitle%5D)+AND+%22J+Biomed+Sci%22%5BJournal%5D)">Spatial multi-omics analyses of the tumor immune microenvironment.</a> J Biomed Sci. 29 (1), 96(2022).</li>
<li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Gdf15+induces+immunosuppression+via+cd48+on+regulatory+t+cells+in+hepatocellular+carcinoma%5BTitle%5D)+AND+%22J+Immunother+Cancer%22%5BJournal%5D)">Gdf15 induces immunosuppression via cd48 on regulatory t cells in hepatocellular carcinoma.</a> J Immunother Cancer. 9 (9), e002787(2021).</li>
<li>Krams, S. M., Schaffert, S., Lau, A. H., Martinez, O. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Applying+mass+cytometry+to+the+analysis+of+lymphoid+populations+in+transplantation%5BTitle%5D)+AND+%22Am+J+Transplant%22%5BJournal%5D)">Applying mass cytometry to the analysis of lymphoid populations in transplantation.</a> Am J Transplant. 17 (8), 1992-1999 (2017).</li>
</ol>

<table><tbody><tr><td>1×PBS</td><td>HyClone</td><td>SH30256.01</td><td></td></tr><tr><td>10×PBS</td><td>HyClone</td><td>SH30256.01</td><td></td></tr><tr><td>100 mm×20 mm tissue-culture-treated culture dish</td><td>Corning</td><td>430167</td><td></td></tr><tr><td>1000 mL pipette tips</td><td>Rainin</td><td>30389218</td><td></td></tr><tr><td>15 mL centrifuge tube</td><td>NEST</td><td>601052</td><td></td></tr><tr><td>200 mL pipette tips</td><td>Rainin</td><td>30389241</td><td></td></tr><tr><td>40 mm nylon cell strainer/70-mm nylon cell strainer</td><td>Falcon</td><td>352340</td><td></td></tr><tr><td>50 mL centrifuge tube</td><td>NEST</td><td>602052</td><td></td></tr><tr><td>70 μm syringe fifilter</td><td>Sangon Biotech</td><td>F613462-9001</td><td></td></tr><tr><td>Anti-Human CCR2 Antibody (clone: K036C2)</td><td>BioLegend</td><td>357224</td><td></td></tr><tr><td>Anti-Human CCR3 Antibody (clone: 5E8)</td><td>BioLegend</td><td>310724</td><td></td></tr><tr><td>Anti-Human CCR7 Antibody (clone: G043H7)</td><td>BioLegend</td><td>353240</td><td></td></tr><tr><td>Anti-Human CD103 Antibody (clone: Ber-ACT8)</td><td>BioLegend</td><td>350202</td><td></td></tr><tr><td>Anti-Human CD115 Antibody (clone: 9-4D2-1E4)</td><td>BioLegend</td><td>347314</td><td></td></tr><tr><td>Anti-Human CD117 Antibody (clone: 104D2)</td><td>BioLegend</td><td>313201</td><td></td></tr><tr><td>Anti-human CD11b Antibody (clone: 1CRF44)&nbsp;</td><td>BD</td><td>562721</td><td></td></tr><tr><td>Anti-human CD11c Antibody (clone: B-ly6)&nbsp;</td><td>BD</td><td>563026</td><td></td></tr><tr><td>Anti-Human CD123 Antibody (clone: 6H6)</td><td>BioLegend</td><td>306002</td><td></td></tr><tr><td>Anti-Human CD127 Antibody (clone: A019D5)</td><td>BioLegend</td><td>351337</td><td></td></tr><tr><td>Anti-Human CD13 Antibody (clone: WM19)</td><td>BioLegend</td><td>301701</td><td></td></tr><tr><td>Anti-Human CD138 Antibody (clone: MI15)</td><td>BioLegend</td><td>356535</td><td></td></tr><tr><td>Anti-human CD14 Antibody (clone: HCD14)</td><td>BioLegend</td><td>325604</td><td></td></tr><tr><td>Anti-Human CD141 Antibody (clone: M80)</td><td>BioLegend</td><td>344102</td><td></td></tr><tr><td>Anti-Human CD15 Antibody (clone: QA19A61)</td><td>BioLegend</td><td>376302</td><td></td></tr><tr><td>Anti-human CD16 Antibody (clone: B7311)&nbsp;</td><td>BD</td><td>561313</td><td></td></tr><tr><td>Anti-Human CD161 Antibody (clone: HP-3G10)</td><td>BioLegend</td><td>339902</td><td></td></tr><tr><td>Anti-Human CD163 Antibody (clone: GHI/61)</td><td>BioLegend</td><td>333603</td><td></td></tr><tr><td>Anti-Human CD169 Antibody (clone: 7-239)</td><td>BioLegend</td><td>346002</td><td></td></tr><tr><td>Anti-human CD19 Antibody (clone: HIB19)</td><td>BioLegend</td><td>302226</td><td></td></tr><tr><td>Anti-Human CD1c Antibody (clone: L161)</td><td>BioLegend</td><td>331501</td><td></td></tr><tr><td>Anti-Human CD20 Antibody (clone: 2H7)</td><td>BioLegend</td><td>302301</td><td></td></tr><tr><td>Anti-Human CD206 Antibody (clone: 15-2)</td><td>BioLegend</td><td>321151</td><td></td></tr><tr><td>Anti-Human CD24 Antibody (clone: ML5)</td><td>BioLegend</td><td>311129</td><td></td></tr><tr><td>Anti-Human CD25 Antibody (clone: BC96)</td><td>BioLegend</td><td>302624</td><td></td></tr><tr><td>Anti-human CD3 Antibody (clone: UCHT1)&nbsp;</td><td>BD</td><td>555916</td><td></td></tr><tr><td>Anti-Human CD31 Antibody (clone: W18200D)</td><td>BioLegend</td><td>375902</td><td></td></tr><tr><td>Anti-Human CD32 Antibody (clone: FUN-2)</td><td>BioLegend</td><td>303232</td><td></td></tr><tr><td>Anti-Human CD326 Antibody (clone: CO17-1A)</td><td>BioLegend</td><td>369812</td><td></td></tr><tr><td>Anti-Human CD33 Antibody (clone: WM53)</td><td>BioLegend</td><td>303402</td><td></td></tr><tr><td>Anti-human CD4 Antibody (clone: L200)&nbsp;</td><td>BD</td><td>563094</td><td></td></tr><tr><td>Anti-Human CD45 Antibody (clone: HI30)&nbsp;</td><td>BD</td><td>563716</td><td></td></tr><tr><td>Anti-Human CD45RO Antibody (clone: UCHL1)</td><td>BioLegend</td><td>304220</td><td></td></tr><tr><td>Anti-human CD56 Antibody (clone: 5.1H11)</td><td>BioLegend</td><td>362510</td><td></td></tr><tr><td>Anti-Human CD64 Antibody (clone: S18012C)</td><td>BioLegend</td><td>399502</td><td></td></tr><tr><td>Anti-Human CD66b Antibody (clone: 6/40c)</td><td>BioLegend</td><td>392917</td><td></td></tr><tr><td>Anti-human CD68 Antibody (clone: Y1/82A)</td><td>BioLegend</td><td>333808</td><td></td></tr><tr><td>Anti-Human CD69 Antibody (clone: FN50)</td><td>BioLegend</td><td>310902</td><td></td></tr><tr><td>Anti-Human CD7 Antibody (clone: 4H9/CD7)</td><td>BioLegend</td><td>395602</td><td></td></tr><tr><td>Anti-human CD8 Antibody (clone: RPA-T8)&nbsp;</td><td>BD</td><td>557750</td><td></td></tr><tr><td>Anti-Human CD80 Antibody (clone: W17149D)</td><td>BioLegend</td><td>375402</td><td></td></tr><tr><td>Anti-Human CD86 Antibody (clone: BU63)</td><td>BioLegend</td><td>374202</td><td></td></tr><tr><td>Anti-Human FOXP3 Antibody (clone: 206D)</td><td>BioLegend</td><td>320101</td><td></td></tr><tr><td>Anti-Human HLA_ABC Antibody (clone: W6/32)</td><td>BioLegend</td><td>311426</td><td></td></tr><tr><td>Anti-human HLA-DR Antibody (clone: L243)</td><td>BioLegend</td><td>307650</td><td></td></tr><tr><td>Anti-Human IgD Antibody (clone: IA6-2)</td><td>BioLegend</td><td>348211</td><td></td></tr><tr><td>Anti-Human Ki67 Antibody (clone: Ki-67)</td><td>BioLegend</td><td>350501</td><td></td></tr><tr><td>Anti-Human PD_L2 Antibody (clone: MH22B2)</td><td>BD</td><td>567783</td><td></td></tr><tr><td>Anti-Human PD1 Antibody (clone: EH12.2H7)</td><td>BioLegend</td><td>329951</td><td></td></tr><tr><td>Anti-Human PDL1 Antibody (clone: MIH2)</td><td>BioLegend</td><td>393602</td><td></td></tr><tr><td>Anti-human TCR-γδ Antibody (clone: B1)&nbsp;</td><td>BD</td><td>740415</td><td></td></tr><tr><td>Cell cryopreservation

간세포암(hepatocellular carcinoma)의 전신 및 국소 면역 반응에 대한 질량 세포 분석

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

간세포암(Hepatocellular Carcinoma)의 전신 및 국소 면역 반응에 대한 질량 세포 분석

Hepatocellular carcinoma (HCC) is one of the most common and deadliest forms of liver cancer worldwide. Despite advances in treatment, the prognosis for ...

mass-cytometry-analysis-systemic-local-immune-responses

Research

연구

JoVE Journal

Biology

생물학

여기 우리가 설립된 피하 종양에서 종양 면역 및 비 면역 microenvironment의 구성 요소를 풍부 하 게 분리 하는 방법을 설명 합니다. 이 기술은 종양 면역 침투 및 비 면역 종양 분수는 종양 면역 microenvironment의 포괄적인 특성화를 허용할 수 있는 별도 분석에 대 한 수 있습니다.

농축 및 설립된 피하 Murine 종양에서 종양 면역 및 비 면역 Microenvironments의 특성

여기 우리 면역 phenotypic와 기능 (세포내 cytokine 유도)를 평가 하는 단일 셀 proteomic 접근 방식을 설명 주변 전체 혈액 샘플, 대량 cytometry 통해 분석으로 변경.

Immunophenotype 및 Cytokine 생산 대량 Cytometry 통해 주변 전 혈의 단일 세포 분석

여기서, 간세포암(HCC)의 전형적인 면역 특징을 재기하는 간암의 임상적으로 관련 있는 뮤린 모델의 개발을 설명한다.

간 염증과 섬유증의 설정에서 발생하는 간세포암의 종양 성 간세포 유도 직교 마우스 모델

암세포에 의한 자연살해(NK) 세포 매개 박멸의 회피는 암 개시 및 진행에 중요하다. 여기에서, 우리는 간 종양 세포를 향해 NK 세포 매개 세포 독성을 평가하기 위하여 2개의 비 방사능 기지를 둔 프로토콜을 제시합니다. 또한 NK 세포 마이그레이션을 분석하기 위해 세 번째 프로토콜이 제시됩니다.

간 종양 세포의 맥락에서 자연 킬러 세포 매개 세포 독성 및 이동의 측정

여기에서는, 우리는 포르말린 고정된 파라핀 내장된 종양 조직 단면도에 있는 면역 세포를 구상하고, 정량화하고, 매핑하기 위한 간단하고 접근가능한 전략을 기술합니다. 이 방법론은 기존의 이미징 및 디지털 분석 기법과 이미징 분석의 다중 분석 기능 및 다중 매개 변수 분석을 확장하는 목적으로 결합합니다.

종양 미세 환경에서 면역 세포 집단의 시각화, 정량화 및 매핑

관련 인간 질병을 충실하게 재구성하는 포괄적인 체외 모델은 부족합니다. 현재 연구는 인간 간세포 암에서 종양 기질 상호 작용을 연구하는 신뢰할 수있는 체외 도구인 3차원 (3D) 종양 스페로이드 생성 및 문화를 제시합니다.

간세포 암에서 종양-기질 상호 작용을 조사하는 3차원 스페로이드 모델

이 프로토콜은 면역 종양학 연구를 위한 인간 면역 체계(HIS) 마우스의 생성을 간략하게 설명합니다. 이 모델에 이식된 인간 종양에 대한 인간 면역치료제를 시험하기 위한 이 모델의 사용에 있어서의 지침 및 고려사항은 종양에 대한 인간 면역계의 반응을 특성화하는 것에 중점을 두고 제시된다.

인간 종양을 지닌 인간화 마우스 모델에서 암 면역 치료제 테스트

여기에서는 암-면역 주기를 구성하는 단계를 모니터링하고 특성화하기 위한 간단하고 빠르며 신뢰할 수 있는 다중 파라메트릭 유세포 분석 및 종양 온 칩 지원 프로토콜에 대해 자세히 설명합니다. 실제로, 암과 면역 세포 간의 상호 작용에 대한 철저한 이해는 종양을 능가하고 임상 치료를 안내하는 데 중요한 통찰력을 제공합니다.

암-면역 주기를 재현하기 위한 Co-Culture In Vitro 시스템

절제 후 간세포암의 내측 가장자리와 종양 주위 영역에 비만세포가 존재하면 유리한 예후를 얻을 수 있습니다. 이 연구는 QuPath 이미지 분석 소프트웨어를 디지털 병리학의 재현성, 일관성 및 정확성에 대한 요구를 충족할 수 있는 유망한 플랫폼으로 승인합니다.

간세포 암종의 미세환경에서의 비만 세포는 유리한 예후를 제공합니다: QuPath 이미지 분석 소프트웨어를 사용한 후향적 연구 

이 동영상은 절개와 태아 thymus의 제거뿐만 아니라 2 dGuo - 처리 thymus의 전 생체내 문화의 준비를 보여줍니다.

2 dGuo - 치료 Thymus 오르간 문화의 준비

학습하세요 간세포암의 전신 및 국소 면역 반응을 질량 세포 분석(CyTOF)으로 평가하는 방법을. 단일 세포 수준에서 면역 세포를 프로파일링하여 14개의 독특한 세포 유형을 식별하고 종양 진행과 관련된 면역 회피 메커니즘을 이해할 수 있습니다.

간세포암(Hepatocellular Carcinoma)의 전신 및 국소 면역 반응에 대한 질량 세포 분석

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