Name: Preparation of intact tissue for microscopic analysis of the endosperm cell layer in Developing and mature Arabidopsis seeds(개발 및 성숙한 애기장대 종자에서 배유 세포층의 현미경 분석을 위한 온전한 조직의 준비) (동영상) | JoVE Journal
Uploaded: 2025-05-16T14:40:00.000+00:00
Duration: 6 min 28 s
Description: 배우세요 Arabidopsis thaliana 종자의 배유 세포층을 화학적 고정 없이 온전하게 준비하는 방법을. 형광 생세포 현미경을 통해 배유 역학을 고해상도로 관찰하여 종자 발아의 분자 메커니즘을 규명할 수 있습니다.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Bentsink, L., Jowett, J., Hanhart, J. C., Koornneef, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cloning+of+DOG1%2C+a+quantitative+trait+locus+controlling+seed+dormancy+in+Arabidopsis%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+USA%22%5BJournal%5D)">Cloning of DOG1, a quantitative trait locus controlling seed dormancy in Arabidopsis.</a> Proc Natl Acad Sci USA. 103 (45), 17042-17047 (2006).</li>
<li>Toh, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High+temperature-induced+abscisic+acid+biosynthesis+and+its+role+in+the+inhibition+of+gibberellin+action+in+Arabidopsis+seeds%5BTitle%5D)+AND+%22Plant+Physiol%22%5BJournal%5D)">High temperature-induced abscisic acid biosynthesis and its role in the inhibition of gibberellin action in Arabidopsis seeds.</a> Plant Physiol. 146 (3), 1368-1385 (2008).</li>
<li>Seo, M., Nambara, E., Choi, G., Yamaguchi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Interaction+of+light+and+hormone+signals+in+germinating+seeds%5BTitle%5D)+AND+%22Plant+Mol+Biol%22%5BJournal%5D)">Interaction of light and hormone signals in germinating seeds.</a> Plant Mol Biol. 69 (4), 463-472 (2009).</li>
<li>Shinomura, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Action+spectra+for+phytochrome+A-+and+B-specific+photoinduction+of+seed+germination+in+Arabidopsis+thaliana%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+USA%22%5BJournal%5D)">Action spectra for phytochrome A- and B-specific photoinduction of seed germination in Arabidopsis thaliana.</a> Proc Natl Acad Sci USA. 93 (15), 8129-8133 (1996).</li>
<li>Liu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nitric+oxide-induced+rapid+decrease+of+abscisic+acid+concentration+is+required+in+breaking+seed+dormancy+in+Arabidopsis%5BTitle%5D)+AND+%22New+Physiol%22%5BJournal%5D)">Nitric oxide-induced rapid decrease of abscisic acid concentration is required in breaking seed dormancy in Arabidopsis.</a> New Physiol. 183 (4), 1030-1042 (2009).</li>
<li>Yan, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((NIN-like+protein+8+is+a+master+regulator+of+nitrate-promoted+seed+germination+in+Arabidopsis%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis.</a> Nat Commun. 7, 13179(2016).</li>
<li>Yan, D., Duermeyer, L., Leoveanu, C., Nambara, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+functions+of+the+endosperm+during+seed+germination%5BTitle%5D)+AND+%22Plant+Cell+Physiol%22%5BJournal%5D)">The functions of the endosperm during seed germination.</a> Plant Cell Physiol. 55 (9), 1521-1533 (2014).</li>
<li>Lopes, A. M., Larkins, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Endosperm+origin%2C+development%2C+and+function%5BTitle%5D)+AND+%22Plant+Cell%22%5BJournal%5D)">Endosperm origin, development, and function.</a> Plant Cell. 5 (10), 1383-1399 (1993).</li>
<li>Penfield, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Reserve+mobilization+in+the+Arabidopsis+endosperm+fuels+hypocotyl+elongation+in+the+dark%2C+is+independent+of+abscisic+acid%2Cand+requires+PHOSPHOENOLPYRUVATE+CARBOXYKINASE1%5BTitle%5D)+AND+%22Plant+Cell%22%5BJournal%5D)">Reserve mobilization in the Arabidopsis endosperm fuels hypocotyl elongation in the dark, is independent of abscisic acid,and requires PHOSPHOENOLPYRUVATE CARBOXYKINASE1.</a> Plant Cell. 16 (10), 2705-2718 (2004).</li>
<li>Leubner-Metzger, G., Fründt, C., Meins, F. Jr <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effects+of+gibberellins%2C+darkness+and+osmotica+on+endosperm+rupture+and+class+I+%CE%B2-1%2C3-glucanase+induction+in+tobacco+seed+germination%5BTitle%5D)+AND+%22Planta%22%5BJournal%5D)">Effects of gibberellins, darkness and osmotica on endosperm rupture and class I β-1,3-glucanase induction in tobacco seed germination.</a> Planta. 199, 282-288 (1996).</li>
<li>Sargant, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+work+on+the+results+of+fertilization+in+angiosperms%5BTitle%5D)+AND+%22Ann+Bot%22%5BJournal%5D)">Recent work on the results of fertilization in angiosperms.</a> Ann Bot. 14 (4), 689-712 (1900).</li>
<li>Groot, P. S., Karssen, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Gibberellins+regulate+seed+germination+in+tomato+by+endosperm+weakening%3A+A+study+with+gibberellin-deficient+mutants%5BTitle%5D)+AND+%22Planta%22%5BJournal%5D)">Gibberellins regulate seed germination in tomato by endosperm weakening: A study with gibberellin-deficient mutants.</a> Planta. 171 (4), 525-531 (1987).</li>
<li>Groot, P. S., Kieliszewa-Rokicka, B., Vermeer, E., Karssen, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Gibberellin-induced+hydrolysis+of+endosperm+cell+walls+in+gibberellin-deficient+tomato+seeds+prior+to+radicle+protrusion%5BTitle%5D)+AND+%22Planta%22%5BJournal%5D)">Gibberellin-induced hydrolysis of endosperm cell walls in gibberellin-deficient tomato seeds prior to radicle protrusion.</a> Planta. 174, 500-504 (1988).</li>
<li>Lee, P. K., Piskurewicz, U., Turečková, V., Strnad, M., Lopez-Molina, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+seed+coat+bedding+assay+shows+that+RGL2-dependent+release+of+abscisic+acid+by+the+endosperm+controls+embryo+growth+in+Arabidopsis+dormant+seeds%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+USA%22%5BJournal%5D)">A seed coat bedding assay shows that RGL2-dependent release of abscisic acid by the endosperm controls embryo growth in Arabidopsis dormant seeds.</a> Proc Natl Acad Sci USA. 107 (44), 19108-19113 (2010).</li>
<li>Lee, P. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spatially+and+genetically+distinct+control+of+seed+germination+by+phytochromes+A+and+B%5BTitle%5D)+AND+%22Genes+Dev%22%5BJournal%5D)">Spatially and genetically distinct control of seed germination by phytochromes A and B.</a> Genes Dev. 26 (17), 1984-1996 (2012).</li>
<li>Piskurewicz, U., Sentandreu, M., Iwasaki, M., Glauser, G., Lopez-Molina, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+Arabidopsis+endosperm+is+a+temperature-sensing+tissue+that+implements+seed+thermoinhibition+through+phyB%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">The Arabidopsis endosperm is a temperature-sensing tissue that implements seed thermoinhibition through phyB.</a> Nat Commun. 14, 1202(2023).</li>
<li>Shinozaki, D., Takayama, E., Kawakami, N., Yoshimoto, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Autophagy+maintains+endosperm+quality+during+seed+storage+to+preserve+germination+ability+in+Arabidopsis%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+USA%22%5BJournal%5D)">Autophagy maintains endosperm quality during seed storage to preserve germination ability in Arabidopsis.</a> Proc Natl Acad Sci USA. 121 (14), e2321612121(2024).</li>
<li>Littlejohn, R. G., Gouveia, D. J., Edner, C., Smirnoff, N., Love, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perfluorodecalin+enhances+in+vivo+confocal+microscopy+resolution+of+Arabidopsis+thaliana+mesophyll%5BTitle%5D)+AND+%22New+Phytol%22%5BJournal%5D)">Perfluorodecalin enhances in vivo confocal microscopy resolution of Arabidopsis thaliana mesophyll.</a> New Phytol. 186 (4), 1018-1025 (2010).</li>
<li>Littlejohn, R. G., Love, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+simple+method+for+imaging+Arabidopsis+leaves+using+perfluorodecalin+as+an+infiltrative+imaging+medium%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">A simple method for imaging Arabidopsis leaves using perfluorodecalin as an infiltrative imaging medium.</a> J Vis Exp. (59), e3394(2012).</li>
<li>Chen, M., Schwab, R., Chory, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+the+requirements+for+localization+of+phytochrome+B+to+nuclear+bodies%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+USA%22%5BJournal%5D)">Characterization of the requirements for localization of phytochrome B to nuclear bodies.</a> Proc Natl Acad Sci USA. 100 (24), 14493-14498 (2003).</li>
<li>Matsushita, T., Mochizuki, N., Nagatani, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dimers+of+the+N-terminal+domain+of+phytochrome+B+are+functional+in+the+nucleus%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Dimers of the N-terminal domain of phytochrome B are functional in the nucleus.</a> Nature. 424 (6948), 571-574 (2003).</li>
<li>Buskirk, V. K. E., Decker, V. P., Chen, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Photobodies+in+light+signaling%5BTitle%5D)+AND+%22Plant+Physiol%22%5BJournal%5D)">Photobodies in light signaling.</a> Plant Physiol. 158 (1), 52-60 (2012).</li>
<li>Logan, C. D., Leaver, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mitochondria-targeted+GFP+highlights+the+heterogeneity+of+mitochondrial+shape%2C+size+and+movement+within+living+plant+cells%5BTitle%5D)+AND+%22J+Exp+Bot%22%5BJournal%5D)">Mitochondria-targeted GFP highlights the heterogeneity of mitochondrial shape, size and movement within living plant cells.</a> J Exp Bot. 51 (346), 865-871 (2000).</li>
<li>Paszkiewicz, G., Gualberto, M. J., Benamar, A., Macherel, D., Logan, C. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Arabidopsis+seed+mitochondria+are+bioenergetically+active+immediately+upon+imbibition+and+specialize+via+biogenesis+in+preparation+for+autotrophic+growth%5BTitle%5D)+AND+%22Plant+Cell%22%5BJournal%5D)">Arabidopsis seed mitochondria are bioenergetically active immediately upon imbibition and specialize via biogenesis in preparation for autotrophic growth.</a> Plant Cell. 29 (1), 109-128 (2017).</li>
<li>Lefebvre, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Functional+analysis+of+Arabidopsis+NCED6+and+NCED9+genes+indicates+that+ABA+synthesized+in+the+endosperm+is+involved+in+the+induction+of+seed+dormancy%5BTitle%5D)+AND+%22Plant+J%22%5BJournal%5D)">Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy.</a> Plant J. 45 (3), 309-319 (2006).</li>
<li>Okamoto, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((CYP707A1+and+CYP707A2%2C+which+encode+abscisic+acid+8%27+hydroxylases%2C+are+indispensable+for+proper+control+of+seed+dormancy+and+germination+in+Arabidopsis%5BTitle%5D)+AND+%22Plant+Physiol%22%5BJournal%5D)">CYP707A1 and CYP707A2, which encode abscisic acid 8' hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis.</a> Plant Physiol. 141 (1), 97-107 (2006).</li>
<li>Endo, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tissue-specific+transcriptome+analysis+reveals+cell+wall+metabolism%2C+flavonol+biosynthesis+and+defense+responses+are+activated+in+the+endosperm+of+germinating+Arabidopsis+thaliana+seeds%5BTitle%5D)+AND+%22Plant+Cell+Physiol%22%5BJournal%5D)">Tissue-specific transcriptome analysis reveals cell wall metabolism, flavonol biosynthesis and defense responses are activated in the endosperm of germinating Arabidopsis thaliana seeds.</a> Plant Cell Physiol. 53 (1), 16-27 (2012).</li>
<li>Lee, P. K., Lopez-Molina, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+seed+coat+bedding+assay+to+genetically+explore+in+vitro+how+the+endosperm+controls+seed+germination+in+Arabidopsis+thaliana%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">A seed coat bedding assay to genetically explore in vitro how the endosperm controls seed germination in Arabidopsis thaliana.</a> J Vis Exp. (81), e50732(2013).</li>
<li>Uno, K., Sugimoto, N., Sato, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((N-aryl+pyrido+cyanine+derivatives+are+nuclear+and+organelle+DNA+markers+for+two-photon+and+super-resolution+imaging%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">N-aryl pyrido cyanine derivatives are nuclear and organelle DNA markers for two-photon and super-resolution imaging.</a> Nat Commun. 12, 2650(2021).</li>
<li>Shinozaki, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Autophagy+increases+zinc+bioavailability+to+avoid+light-mediated+reactive+oxygen+species+production+under+zinc+deficiency%5BTitle%5D)+AND+%22Plant+Physiol%22%5BJournal%5D)">Autophagy increases zinc bioavailability to avoid light-mediated reactive oxygen species production under zinc deficiency.</a> Plant Physiol. 182 (3), 1284-1296 (2020).</li>
<li>Laxmi, A., Pan, J., Morsy, M., Chen, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Light+plays+an+essential+role+in+intracellular+distribution+of+auxin+efflux+carrier+PIN2+in+Arabidopsis+thaliana%5BTitle%5D)+AND+%22PLOS+One%22%5BJournal%5D)">Light plays an essential role in intracellular distribution of auxin efflux carrier PIN2 in Arabidopsis thaliana.</a> PLOS One. 3 (1), e1510(2008).</li>
<li>Rigal, A., Doyle, M. S., Robert, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Live+cell+imaging+of+FM4-64%2C+a+tool+for+tracing+the+endocytic+pathways+in+Arabidopsis+root+cells%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Live cell imaging of FM4-64, a tool for tracing the endocytic pathways in Arabidopsis root cells.</a> Methods Mol Biol. 1242, 93-103 (2015).</li>
<li>Shimomura, O., Johnson, H. F., Saiga, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Extraction%2C+purification+and+properties+of+Aequorin%2C+a+bioluminescent+protein+from+the+luminous+hydromedusan%2C+Aequorea%5BTitle%5D)+AND+%22J+Cell+Comp+Physiol%22%5BJournal%5D)">Extraction, purification and properties of Aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea.</a> J Cell Comp Physiol. 59 (3), 223-239 (1962).</li>
<li>Holzinger, A., Buchner, O., Lütz, C., Hanson, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Temperature-sensitive+formation+of+chloroplast+protrusions+and+stromules+in+mesophyll+cells+of+Arabidopsis+thaliana%5BTitle%5D)+AND+%22Protoplasma%22%5BJournal%5D)">Temperature-sensitive formation of chloroplast protrusions and stromules in mesophyll cells of Arabidopsis thaliana.</a> Protoplasma. 230, 23-30 (2007).</li>
<li>Mano, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Distribution+and+characterization+of+peroxisomes+in+Arabidopsis+by+visualization+with+GFP%3A+dynamic+morphology+and+actin-dependent+movement%5BTitle%5D)+AND+%22Plant+Cell+Physiol%22%5BJournal%5D)">Distribution and characterization of peroxisomes in Arabidopsis by visualization with GFP: dynamic morphology and actin-dependent movement.</a> Plant Cell Physiol. 43 (3), 331-341 (2002).</li>
<li>Nelson, K. B., Cai, X., Nebenführ, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+multicolored+set+of+in+vivo+organelle+markers+for+co-localization+studies+in+Arabidopsis+and+other+plants%5BTitle%5D)+AND+%22Plant+J%22%5BJournal%5D)">A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants.</a> Plant J. 51 (6), 1126-1136 (2007).</li>
<li>Goto, C., Tamura, K., Fukao, Y., Shimada, T., Hara-Nishimura, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+novel+nuclear+envelope+protein+KAKU4+modulates+nuclear+morphology+in+Arabidopsis%5BTitle%5D)+AND+%22Plant+Cell%22%5BJournal%5D)">The novel nuclear envelope protein KAKU4 modulates nuclear morphology in Arabidopsis.</a> Plant Cell. 26 (5), 2143-2155 (2014).</li>
<li>Geldner, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((combinatorial+analysis+of+membrane+compartments+in+intact+plants+with+a+multicolor+marker+set%5BTitle%5D)+AND+%22Plant+J%22%5BJournal%5D)">combinatorial analysis of membrane compartments in intact plants with a multicolor marker set.</a> Plant J. 59 (1), 169-178 (2009).</li>
<li>Jang, I. -C., Henriques, R., Seo, S. H., Nagatani, A., Chua, N. -H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Arabidopsis+phytochrome+interacting+factor+proteins+promote+phytochrome+B+polyubiquitination+by+COP1+E3+ligase+in+the+nucleus%5BTitle%5D)+AND+%22Plant+Cell%22%5BJournal%5D)">Arabidopsis phytochrome interacting factor proteins promote phytochrome B polyubiquitination by COP1 E3 ligase in the nucleus.</a> Plant Cell. 22 (7), 2370-2383 (2010).</li>
<li>AI-Sady, B., Ni, W., Kircher, S., Schäfer, E., Quail, H. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Photoactivated+phytochrome+induces+rapid+PIF3+phosphorylation+prior+to+proteasome-mediated+degradation%5BTitle%5D)+AND+%22Mol+Cell%22%5BJournal%5D)">Photoactivated phytochrome induces rapid PIF3 phosphorylation prior to proteasome-mediated degradation.</a> Mol Cell. 23 (4), 439-446 (2006).</li>
<li>Lam, K. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((BFA-induced+compartments+from+the+Golgi+apparatus+and+trans-Golgi+network%2Fearly+endosome+are+distinct+in+plant+cells%5BTitle%5D)+AND+%22Plant+J%22%5BJournal%5D)">BFA-induced compartments from the Golgi apparatus and trans-Golgi network/early endosome are distinct in plant cells.</a> Plant J. 60 (5), 865-881 (2009).</li>
<li>Yoshimoto, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Processing+of+ATG8s%2C+ubiquitin-like+proteins%2C+and+their+deconjugation+by+ATG4s+are+essential+for+plant+autophagy%5BTitle%5D)+AND+%22Plant+Cell%22%5BJournal%5D)">Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy.</a> Plant Cell. 16 (11), 2967-2983 (2004).</li>
<li>Xu, G., Zhang, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mechanisms+controlling+seed+size+by+early+endosperm+development%5BTitle%5D)+AND+%22Seed+Biol%22%5BJournal%5D)">Mechanisms controlling seed size by early endosperm development.</a> Seed Biol. 2, 1(2023).</li>
</ol>

<table><tbody><tr><td>1.5 mL 미세 원심분리기 튜브</td><td>Watoson Bio Lab</td><td>131-815C</td><td></td></tr><tr><td>커버슬립 (18 x 18 mm)</td> <td>Matsunami Glass Ind.,Ltd.</td><td>C218181</td><td></td></tr><tr><td>장착용</td><td>DDW</td><td></td><td></td> 물</tr> 여<tr><td>과지 No.526 (400 x 400 mm)</td><td>ADVANTEC VIETNAM CO., LTD.</td><td>02453400&nbsp;</td><td></td></tr><tr><td>겐키군 세루 센요 요도 코프 N-150 (55L)</td> <td>카타쿠라 & Co-op Agri Corporation</td><td></td><td>식물 성장용 토양</td></tr><tr><td>유리 슬라이드 (26mm x 76mm)</td><td>Matsunami Glass Ind.,Ltd.</td><td>S1215</td><td></td></tr><tr><td>Grodan AO 36 x 36 x 40 mm 큐브</td> 식물 성장 아이리스용 <td>Grodan</td><td></td><td>Rockwools</td></tr><tr><td>&nbsp; 가위</td><td>프리미엄 플러스&nbsp; 일본 주식회사</td><td>FC-0212</td><td></td></tr><tr><td>보석상 집게, Dumont No. 5(4 1/4인치)</td>라이 <td></td> 카 어플리케이션 제품군 X(LAS X)&nbsp <td>찢어짐용</td>Dumont <td>F6521</td> 집게</tr><tr><td>;</td><td>Sterallis 8용</td> <td>라이카</td><td></td> 소프트웨어</tr> 라이<tr><td>카 마이크로시스템즈&nbsp; 현미경용 침지 오일 매우</td><td>낮은 자가형광 침지 오일</td><td>THMOIL-10LF</td><td></td></tr><tr><td>LIOR 정밀 겸자 110mm&nbsp; SL-14</td><td>케니스 주식회사</td><td>KN33450438</td><td>홀딩용 집게&nbsp;</td></tr><tr><td>NAIL HOLIC</td><td>KOSE</td><td></td><td>매니큐어</td></tr><tr><td>바늘 27G 3/4 (19 mm) RB&nbsp;</td><td>미사와 메디컬 산업 주식회사</td><td></td><td></td></tr><tr><td>Nichipet Air 1000 uL</td><td>Nichiryo</td><td>00-NAR-1000</td><td>A 1000 및 micro 절단용 Ingection Needle; L 마이크로피펫</td></tr><tr><td>퍼플루오로데칼린</td><td>APOLLO SCIENTIFIC</td><td>PC5960</td><td></td></tr><tr><td>적색광/원적외선 LED 패널</td> 장착용 시약 <td>TOKYO RIKAKIKAI CO., LTD.</td><td>10147599</td><td></td></tr><tr><td>Schappe Spun #60</td><td>Fujix Co., Ltd.</td><td></td><td>스레드</td></tr><tr><td>SPINKOTE 윤활제 2온스</td><td>BECKMAN COULTER</td><td>306812</td><td>그리스</td></tr><tr><td>Sterallis 8</td><td>라이카</td><td></td><td>공초점 레이저 주사 현미경</td></tr><tr><td>실체 현미경 Stemi 305 캠 W</td><td>Carl Zeiss NTS Ltd.</td><td>491903-0017-000</td><td></td></tr><tr><td>백색광 LED</td><td>PANASONIC</td><td>FL40SSW/37</td><td></td></tr></tbody></table>

preparation of intact tissue for microscopic analysis of the endosperm cell layer in developing and mature arabidopsis seeds(개발 및 성숙한 애기장대 종자에서 배유 세포층의 현미경 분석을 위한 온전한 조직의 준비)

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

그림 1에 표시된 프로토콜을 사용하여 14 DAF에서 실리크에서 수확한 종자를 개발하여 배유 샘플을 준비했습니다(이 단계에서 testa는 여전히 녹색입니다). 넓은 지역에 걸쳐 수많은 배유 세포와 세포 내 구조가 관찰되었습니다(그림 3A). 이 실험에서는 C-말단에서 GFP와 융합된 PHYB를 발현하는 종자(PHYB-GFP)를 사용했습니다. PHYB는 적색광에 의해 활성화될 때 핵으로 전위되고 종자 발아에 관여하는 광체(PB)로 알려진 PHYB 양성 반점을 형성한다는 것은 잘 알려져 있습니다 4,20,21,22. 샘플은 22°C에서 연속적인 백색광 조건에서 자란 식물에서 수확했습니다. <stro...

Preparation of intact tissue for microscopic analysis of the endosperm cell layer in Developing and mature Arabidopsis seeds(개발 및 성숙한 애기장대 종자에서 배유 세포층의 현미경 분석을 위한 온전한 조직의 준비)

In Arabidopsis seeds, the endosperm, a single layer of living cells located between the embryo and the testa, plays a critical role in regulating seed ...

preparation-intact-tissue-for-microscopic-analysis-endosperm-cell

Research

연구

JoVE Journal

Biology

생물학

각인은 식물과 동물의 복제에 현상입니다. DNA의 methylation은 각인의 메커니즘에서 중요한 역할을합니다. 배젖를 분리 및 각인 유전자의 methylation 상태를...

에 각인 유전자의 DNA Methylation의 결정 Arabidopsis 배젖

우리는 stomatal 차별화를 문서화, 개발 며칠 동안 표피의 공 촛점 이미징을위한 식물 cotyledons를 고정하기 위해 챔버 슬라이드 및 미디어를 사용하여 프로토콜을 설명합니다. 형광 태그 단백질은 세포 분열과 세포 형 차별화 중에 가능한 역할의 이해를 증가 표현과 subcellular 지방화에 의해 동적으로 추적 할 수 있습니다.

의 장기, 고해상도 공 촛점 시간 경과 영상<em&gt; Arabidopsis 떡잎</em발아시&gt; 표피

우리의 개발 초기 단계에서 배아를 분리하는 효율적이고 간단한...

에서 초기 단계의 배아의 효율적이고 신속한 분리<em&gt; 애기 장대</em&gt; 씨앗

우리는 애기 장대 씨앗에서 씨앗 코트 침구 분석 (SCBA)를 조립하는 절차를 제시한다. SCBA는 유전자와 체외에서 탐색 할 수있는 강력한 도구가 될 수 있도록 표시 방식 휴면 종자의 빛 신호에 대한 응답으로 배유 컨트롤 종자 발아. SCBA는 배유가 배아의 성장에 영향을 미칠 것으로 의심되는 모든 상황에서 적용 할 수있는 원리입니다.

유전자 탐구하는 종피 침구 분석<em&gt; 체외</em&gt; 어떻게 배젖 컨트롤 종자 발아에<em&gt; 애기 장대</em

우리는 면역 염색, 형광 제자리 교잡, 전체 마운트 애기 장대 난자의 양적, 고해상도 이미징 다음 DNA 염색 여기를 효율적이고 신뢰할 수있는 프로토콜을 제공합니다. 이 방법은 성공적 염색질 변형 및 핵 구조를 분석하는데 사용 하였다.

전체 마운트 난자에있는 염색질 수정 및 핵 구조의 정량, 단일 세포 분석을위한 효율적인 방법<em&gt; 애기 장대</em

결정질 셀룰로오스는 식물 세포벽의 중요한 구성 성분입니다. 그러나 세포 분해능에서 정량화하는 것은 기술적으로 어렵습니다.  여기에서는 시공간 해상도에서 세포벽 구성에 대한 정보를 얻기 위해 편광 기술과 뿌리 단면을 사용하는 방법을 보고합니다.

에서 결정 셀룰로오스 축적의 높은 해상도 정량<em&gt; 애기 장대</em&gt; 뿌리 조직 특이 세포 벽 수정을 모니터링하는

이 프로토콜 embryogenesis 애기 에 난 허가 뒤에 현미경으로 배아 패턴 형성의 검사를 통해 관찰 하는 방법을 설명 합니다.

애기 에서 Embryogenesis를 특성화 하는 방법

이 프로토콜에서 우리 애기 꽃과 siliques, 몇 가지 기본적인 개간 기술의 적절 한 해 부에 대 한 기법을 설명 하 고 생식 구조의 전체 마운트 관측에 대 한 절차를 얼룩이 지기 선정.

전체-마운트 고 애기 꽃 기관 및 Siliques의 얼룩

이 기술은 종자의 다른 지구에 있는 세포, 전분 과립 및 단백질 바디의 관측 그리고 분석을 위한 전체 종자 크기의 수지 단면도의 빠르고 간단한 준비를 허용합니다.

전체 종자 크기의 수지 섹션 및 응용 프로그램을 얻기위한 간단한 건조 단면 방법

여기에서 우리는 난자의 수용성을 증가시키는 Arabidopsis thaliana에서 꽃가루 튜브 안내 및 수신을 관찰하기 위한 SIV(semi-in vitro) 방법의 개선을 설명합니다. 고처리량 SIV 겸 중격 방법은 배우자 마커 라인 및 유전적으로 인코딩된 바이오센서와 결합하여 수정의 동적 과정을 모니터링할 수 있습니다.

Arabidopsis 꽃가루관 수용 및 이중 수정의 실시간 이미징 semi-In vitro cum septum 방법을 사용한

배우세요 Arabidopsis thaliana 종자의 배유 세포층을 화학적 고정 없이 온전하게 준비하는 방법을. 형광 생세포 현미경을 통해 배유 역학을 고해상도로 관찰하여 종자 발아의 분자 메커니즘을 규명할 수 있습니다.

Preparation of intact tissue for microscopic analysis of the endosperm cell layer in Developing and mature Arabidopsis seeds(개발 및 성숙한 애기장대 종자에서 배유 세포층의 현미경 분석을 위한 온전한 조직의 준비) (동영상) | JoVE Journal

Preparation of intact tissue for microscopic analysis of the endosperm cell layer in Developing and mature Arabidopsis seeds(개발 및 성숙한 애기장대 종자에서 배유 세포층의 현미경 분석을 위한 온전한 조직의 준비)

더 많은 동영상 탐색