Name: Bidirectional electrical and Optoelectronic interfaces in healthy and ischemic ex vivo Rat Hearts(건강한 쥐와 허혈성 생체 외 쥐 심장의 양방향 전기 및 광전자 인터페이스) (동영상) | JoVE Journal
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Description: 학습하세요 생체 외 쥐 심장 모델을 활용한 양방향 광전자 및 전기 인터페이스 기술. 나노다공성 탄소 전극과 Langendorff 장치를 이용한 심장 박동 조절 및 허혈성 심근경색 치료 재료 검증 방법을 익히세요.

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</ol>

<table><tbody><tr><td>16 채널 헤드스테이지</td><td>인탄 테크놀로지스</td><td>C3334</td><td></td></tr><tr><td>2,3,5-트리페닐테트라졸륨 클로라이드</td><td>시그마 알드리치</td><td>T8877-5G</td><td>트리페닐테트라졸륨 클로라이드 </td></tr><tr><td>2-프로판올(IPA) </td><td>피셔 사이언티픽</td><td>A451-4</td><td></td></tr><tr><td>635nm 레이저, 500mW</td><td>레이저글로우</td><td>NA</td><td></td></tr><tr><td>아세톤</td><td>피셔 사이언티픽</td><td>A18-4</td><td></td></tr><tr><td>옹스트롬 Evovac 전자빔 증발기</td><td>옹스트롬</td><td>NA</td><td></td></tr><tr><td>AZ 300MIF 개발자</td><td>미세화학</td><td>18441123163</td><td>개발자</td></tr><tr><td>AZ nlof 2020 </td><td>미세화학</td><td>1A002020</td><td></td></tr><tr><td>AZ NMP </td><td>미세화학</td><td>NA</td><td>포토레지스트 리무버</td></tr><tr><td>AZ40XT-11D </td><td>미세화학</td><td>1000300</td><td>포토 레지스트</td></tr><tr><td>버퍼링된 HF</td><td>써모 사이언티픽</td><td>AA44627K2</td><td></td></tr><tr><td>CaCl2.2H2O</td><td>시그마 알드리치</td><td>(C3881)</td><td></td></tr><tr><td>CO2 레이저 </td><td>범용 레이저 시스템</td><td>VLS 4.60</td><td></td></tr><tr><td>전도성 실버 페인트</td><td>펠코</td><td>16062</td><td>전극과 연결 Cu 와이어 사이의 전도성 접착</td></tr><tr><td>0.1mm 캡슐화 된 구리선</td><td>아마존</td><td>https://www.amazon.com/Enameled-Temperature-Resistance-Transformers-Inductors/dp/ B09TNM51S5/ref=asc_df_B09 TNM51S5?mcid=155de761ea4 c389ea86d45ba61a83e8d& 태그 =하이프로드-20& linkCode=df0& hvadid =693675076770& hvpos=& hvnetw =g& HVRand=664358781094905 4431& hvpone=& hvptwo=& hvqmt =& hvdev=c& hvdvcmdl=& hvlocint =& hvlocphy=9021716& hvtargid= pla-1878323158943& PSC=1</td><td></td></tr><tr><td>Digidata 1550 디지타이저</td><td>분자 장치</td><td>NA</td><td></td></tr><tr><td>포도당</td><td>시그마 알드리치</td><td>지7021</td><td></td></tr><tr><td>HBSS, 칼슘 없음, 마그네슘 없음, 페놀 레드 없음</td><td>써모 사이언티픽</td><td>14175095</td><td></td></tr><tr><td>하이델베르크 MLA150 다이렉트 라이터</td><td>하이델베르크</td><td>NA</td><td></td></tr><tr><td>헤파린(1000IU/kg)</td><td>사에이전트 제약</td><td>1009655</td><td></td></tr><tr><td>헴스</td><td>시그마 알드리치</td><td>(H4034)</td><td></td></tr><tr><td>헥산 </td><td>시그마 알드리치</td><td>52750-10ML</td><td>용매</td></tr><tr><td>고압</td><td>피셔 사이언티픽</td><td>A513-500</td><td>미량 금속 등급</td></tr><tr><td>HMDS Vapor Prime 오븐</td><td>수율 엔지니어링 시스템</td><td>NA</td><td></td></tr><tr><td>HNO3</td><td>피셔 사이언티픽</td><td>A509P500</td><td>미량 금속 등급</td></tr><tr><td>핫 라미네이터</td><td>사무실 창고</td><td>L410-A</td><td>다층 캡슐화 </td></tr><tr><td>Intan RHD 시스템</td><td>인탄 테크놀로지스</td><td>C3100</td><td></td></tr><tr><td>iWorx 심전도</td><td>아이웍스</td><td> IA-400D</td><td></td></tr><tr><td>iWorx LVD 프로브</td><td>아이웍스</td><td>BP-100</td><td></td></tr><tr><td>iWorx 프리앰프</td><td>아이웍스</td><td>C-ISO-256 인증</td><td></td></tr><tr><td>케이클</td><td>시그마 알드리치</td><td>(P4504)</td><td></td></tr><tr><td>라텍스 풍선(사이즈 4)</td><td>라드노티</td><td>170404</td><td></td></tr><tr><td>MgCl2.7H2O</td><td>시그마 알드리치</td><td>(M9272)</td><td></td></tr><tr><td>나크롬</td><td>시그마 알드리치</td><td>(S9625)</td><td></td></tr><tr><td>Nafion 117 함유 용액</td><td>시그마 알드리치</td><td>31175-20-9</td><td>나노다공성 탄소패턴용 고정시약</td></tr><tr><td>NaH2PO4</td><td>시그마 알드리치</td><td>(S0751)</td><td></td></tr><tr><td>나오</td><td>시그마 알드리치</td><td>221465</td><td></td></tr><tr><td>PDMS</td><td>피셔 사이언티픽</td><td>NC9285739</td><td></td></tr><tr><td>Plasma-Therm 유도 결합 플라즈마 불화물 에처</td><td>플라즈마 열</td><td>NA</td><td></td></tr><tr><td>PMMA (495 A6) </td><td>미세화학</td><td>M130006 </td><td></td></tr><tr><td>폴리(피로멜리트산 이무수물-Co-4,4'-옥시디아닐린)</td><td>시그마 알드리치</td><td>575801</td><td>PI 전구체</td></tr><tr><td>폴리(에틸렌-코-비닐 아세테이트)</td><td>시그마 알드리치</td><td>437247-250G</td><td>침투용 폴리머</td></tr><tr><td>폴리(비닐알코올)</td><td>시그마 알드리치</td><td>341584-25G</td><td></td></tr><tr><td>폴리에틸렌 테레프탈레이트(PET)</td><td>아마존</td><td>KS-6304-21-11</td><td>유형 D 투명 PET 시트 .0005" 두께 x 27" 너비 x 10피트 길이 1개</td></tr><tr><td>전위차계</td><td>생물 학적</td><td>SP-200</td><td>전기 자극</td></tr><tr><td>저항 오븐</td><td> 파견</td><td>LCC1-15-5</td><td></td></tr><tr><td>주사 전자 현미경</td><td>칼 자이스</td><td>쇠황조롱이</td><td>형태 특성화를 위한 기기</td></tr><tr><td>실리콘 웨이퍼 </td><td>NOVA 전자 재료</td><td>HS39626</td><td></td></tr><tr><td>실리콘 접착제</td><td>WPI</td><td>KWIK-SIL-S</td><td>캡슐화</td></tr><tr><td>붕산나트륨</td><td>시그마 알드리치</td><td>SX0355</td><td></td></tr><tr><td>SOI 웨이퍼</td><td>울트라실</td><td>3-10541</td><td>유형 오리엔트:P/B(100) DT:7+/-1um DR:&#60;. 005 옴cm HT: 300+/-10um HR: 1-30 옴cm 상자: 1um+/-5% UD-13998/UH-13962</td></tr><tr><td>SU-8 2002</td><td>미세화학</td><td>SU-8 2000 시리즈</td><td></td></tr><tr><td>SU-8 현상액</td><td>미세화학</td><td>SU-8 2000 시리즈</td><td></td></tr><tr><td>물티슈 접착제</td><td>3분</td><td>1469SB</td><td></td></tr><tr><td>Whatman 정성 여과지, 1등급</td><td>시그마 알드리치</td><td>WHA1001150</td><td>탄소 패터닝용 기판</td></tr></tbody></table>

bidirectional electrical and optoelectronic interfaces in healthy and ischemic ex vivo rat hearts(건강한 쥐와 허혈성 생체 외 쥐 심장의 양방향 전기 및 광전자 인터페이스)

Bidirectional Electrical and Optoelectronic Interfaces in Healthy and Ischemic Ex Vivo Rat Hearts

Bidirectional electrical and Optoelectronic interfaces in healthy and ischemic ex vivo Rat Hearts(건강한 쥐와 허혈성 생체 외 쥐 심장의 양방향 전기 및 광전자 인터페이스)

Electrophysiological studies are pivotal in exploring interactions between materials and cardiac biointerfaces. Recent advancements have introduced ...

bidirectional-electrical-optoelectronic-interfaces-healthy-ischemic

Research

연구

JoVE Journal

Bioengineering

생명공학

이 문서 potentiometric 염료 (DI - 8 - ANEPPS)를 사용 Langendorff - perfused 쥐 심장의 표면에 광학 이미지 조치 가능성이 운동에 높은 시간적 및 공간적 해상도 기법을 설명합니다.

Langendorff - perfused 랫 하트의 광학 매핑

이 문서는 파노라마 영상 시스템을 사용 Langendorff - perfused 토끼 마음에 광학 매핑 실험을 실시하기위한 기본 절차 및 듀얼 (전압과 칼슘) 이미징 양상을 설명합니다.

Langendorff - perfused 토끼 하트의 Multiparametric 광 매핑

이 연구의 목적은 번역 동물 모델을 사용하여 심장 역학을 조사하는 방법을 확립하는 것이었습니다. 기술된 실험 접근법은 분리되고 손상되지 않은 돼지 심장 모델에서 전기 적 활동을 평가하기 위한 전기 생리학 연구와 함께 이중 방출 광검을 통합합니다.

전 비보 랑엔도르프-퍼블릭 하트의 검문 및 전기생리학 연구

이 프로토콜은 전임상 약물 검사를 위해 인간의 심장 조각을 단면화 및 배양하는 절차를 설명하고 이러한 조각에서 동시에 세포간 전압 및 세포내 칼슘 신호를 기록하기 위한 광학 매핑의 사용을 자세히 설명합니다.

인간 조직형 심장 슬라이스의 이중 전압 및 칼슘 광학 매핑에 의한 전임상 심장 전극생리학 평가

이 문서는 최소 침습 적 접근을 사용하여 쥐에 있는 심근 허혈 및 후속 만성 재관전을 능력을 발휘하는 능률적인 방법을 제시합니다. 또한, 쥐의 좌심실 혈역학 적 기능은 에코카예그래피와 고립 된 작업 심장 방법에 의해 평가됩니다.

쥐의 심근 경색과 격리 된 작업 심장 시스템에 의한 심장 기능 평가를 유도하는 반 최소 침습 적 방법

이 연구는 마이크로 LED 어레이를 사용한 국소 광 자극 및 심 외막 전위의 동시 광학 매핑을 사용하여 트랜스 제닉 채널 로돕신 -2 (ChR2) 마우스의 손상되지 않은 뮤린 심장의 심장 리듬을 제어하는 방법을보고합니다.

쥐 심장에 광유전학적 다중 부위 광자극을 적용하여 고급 심장 리듬 관리

이 프로토콜은 높은 시간 및 공간 분해능으로 막관통 전압 및 세포 내 Ca2+ 과도 현상의 전기 생리학적 측정을 포함하여 카테콜아민성 다형성 심실 빈맥의 영향을 받는 야생형 및 녹인 동물에서 얻은 마우스 심장의 이중 염료 광학 매핑을 소개합니다.

Catecholaminergic Polymorphic Ventricular Tachycardia의 RyR2R2474S Knock-In 마우스의 심장에 대한 이중 염료 광학 매핑

이 프로토콜은 형질전환 마우스 심장에서 내재적 심장 뉴런의 광유전학적 자극 방법을 보여줍니다. 설명된 접근 방식은 생체 외 관류 심장에서 갑작스러운 심장 뉴런 활성화의 동역학과 콜린성 및 카테콜아민성 활성 간의 상호 작용을 조사하는 데 사용됩니다.

절제된 관류 마우스 심장에서 내재적 심장 자율 뉴런의 광유전학적 활성화(Optogenetic Activation of Intrinsic Cardiac Autonomic Neurons in Excised Perfused Mouse Hearts)

조혈을위한 3D 문화 시스템은 인간 코드 혈액과 leukemic 골수 세포를 사용하여 설명합니다. 방법은 세포외 기질 단백질로 코팅 다공성 합성 폴리 우레탄 발판의​​ 사용을 기반으로합니다. 이 발판은 세포의 광범위한 수용하는 적응이다.

정상과 비정상적인 인간 조혈의 전직 생체내 흉내

에 대한 기계적 적응 고분자 나노 복합 재료의 환경 제어 Microtensile 테스트<em&gt; 생체</em&gt; 특성

학습하세요 생체 외 쥐 심장 모델을 활용한 양방향 광전자 및 전기 인터페이스 기술. 나노다공성 탄소 전극과 Langendorff 장치를 이용한 심장 박동 조절 및 허혈성 심근경색 치료 재료 검증 방법을 익히세요.

Bidirectional electrical and Optoelectronic interfaces in healthy and ischemic ex vivo Rat Hearts(건강한 쥐와 허혈성 생체 외 쥐 심장의 양방향 전기 및 광전자 인터페이스) (동영상) | JoVE Journal

Bidirectional electrical and Optoelectronic interfaces in healthy and ischemic ex vivo Rat Hearts(건강한 쥐와 허혈성 생체 외 쥐 심장의 양방향 전기 및 광전자 인터페이스)

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