Name: 자기 입자 이미징을 사용한 폐 섬유증에 대한 이식된 원위 폐 상피 전구 세포의 이미지 유도 생체 내 추적 (동영상) | JoVE Journal
Uploaded: 2025-06-27T14:40:00.000+00:00
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Description: 배우세요 자기 입자 이미징을 사용하여 폐 섬유증 모델에서 이식된 원위 폐 상피 전구 세포를 비침습적으로 실시간 추적하는 방법을. 이 기술은 세포 치료의 분포와 유지를 정량적으로 평가하여 재생 의학 연구를 지원합니다.

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<li>Glassberg, M. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Allogeneic+human+mesenchymal+stem+cells+in+patients+with+idiopathic+pulmonary+fibrosis+via+intravenous+delivery+%28AETHER%29%3A+A+phase+I+safety+clinical+trial%5BTitle%5D)+AND+%22Chest%22%5BJournal%5D)">Allogeneic human mesenchymal stem cells in patients with idiopathic pulmonary fibrosis via intravenous delivery (AETHER): A phase I safety clinical trial.</a> Chest. 151 (5), 971-981 (2017).</li>
<li>Zhao, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adipose-derived+mesenchymal+stem+cell+therapy+for+reverse+bleomycin-induced+experimental+pulmonary+fibrosis%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Adipose-derived mesenchymal stem cell therapy for reverse bleomycin-induced experimental pulmonary fibrosis.</a> Sci Rep. 13 (1), 13183(2023).</li>
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<li>Llontop, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Airway+transplantation+of+adipose+stem+cells+protects+against+bleomycin-induced+pulmonary+fibrosis%5BTitle%5D)+AND+%22J+Investig+Med%22%5BJournal%5D)">Airway transplantation of adipose stem cells protects against bleomycin-induced pulmonary fibrosis.</a> J Investig Med. 66 (4), 739-746 (2018).</li>
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<li>Ortiz-Muñoz, G., Looney, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-invasive+Intratracheal+Instillation+in+Mice%5BTitle%5D)+AND+%22Bio+Protoc%22%5BJournal%5D)">Non-invasive Intratracheal Instillation in Mice.</a> Bio Protoc. 5 (12), e1504(2015).</li>
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<li>Li, Z., Tong, L., Zhao, H., He, Z. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aLi%2C+Z+%22Medical+Imaging+in+Clinical+Practice%22">Medical Imaging in Clinical Practice</a>. Erondu, F. O. , IntechOpen. (2013).</li>
<li>Knopp, T., Gdaniec, N., Möddel, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Magnetic+particle+imaging%3A+from+proof+of+principle+to+preclinical+applications%5BTitle%5D)+AND+%22Phys+Med+Biol%22%5BJournal%5D)">Magnetic particle imaging: from proof of principle to preclinical applications.</a> Phys Med Biol. 62 (14), R124-R178 (2017).</li>
<li>Borgert, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Fundamentals+and+applications+of+magnetic+particle+imaging%5BTitle%5D)+AND+%22J+Cardiovasc+Computed+Tomogr%22%5BJournal%5D)">Fundamentals and applications of magnetic particle imaging.</a> J Cardiovasc Computed Tomogr. 6 (3), 149-153 (2012).</li>
<li>Mohapatra, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Principles+and+applications+of+magnetic+nanomaterials+in+magnetically+guided+bioimaging%5BTitle%5D)+AND+%22Mater+Today+Phys%22%5BJournal%5D)">Principles and applications of magnetic nanomaterials in magnetically guided bioimaging.</a> Mater Today Phys. 32, 101003(2023).</li>
<li>Pablico-Lansigan, M. H., Situ, S. F., Samia, A. C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Magnetic+particle+imaging%3A+advancements+and+perspectives+for+real-time+in+vivo+monitoring+and+image-guided+therapy%5BTitle%5D)+AND+%22Nanoscale%22%5BJournal%5D)">Magnetic particle imaging: advancements and perspectives for real-time in vivo monitoring and image-guided therapy.</a> Nanoscale. 5 (10), 4040-4055 (2013).</li>
<li>Bulte, J. W. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+%26%2334%3Bhot-spot%26%2334%3B+imaging+of+transplanted+stem+cells+using+superparamagnetic+tracers+and+magnetic+particle+imaging%5BTitle%5D)+AND+%22Tomography%22%5BJournal%5D)">Quantitative &#34;hot-spot&#34; imaging of transplanted stem cells using superparamagnetic tracers and magnetic particle imaging.</a> Tomography. 1 (2), 91-97 (2015).</li>
<li>Lemaster, J. E., Chen, F., Kim, T., Hariri, A., Jokerst, J. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+a+trimodal+contrast+agent+for+acoustic+and+magnetic+particle+imaging+of+stem+cells%5BTitle%5D)+AND+%22ACS+Appl+Nano+Mater%22%5BJournal%5D)">Development of a trimodal contrast agent for acoustic and magnetic particle imaging of stem cells.</a> ACS Appl Nano Mater. 1 (3), 1321-1331 (2018).</li>
<li>Shalaby, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Complementary+early-phase+magnetic+particle+imaging+and+late-phase+positron+emission+tomography+reporter+imaging+of+mesenchymal+stem+cells+in+vivo%5BTitle%5D)+AND+%22Nanoscale%22%5BJournal%5D)">Complementary early-phase magnetic particle imaging and late-phase positron emission tomography reporter imaging of mesenchymal stem cells in vivo.</a> Nanoscale. 15 (7), 3408-3418 (2023).</li>
<li>Wang, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Artificially+engineered+cubic+iron+oxide+nanoparticle+as+a+high-performance+magnetic+particle+imaging+tracer+for+stem+cell+tracking%5BTitle%5D)+AND+%22ACS+Nano%22%5BJournal%5D)">Artificially engineered cubic iron oxide nanoparticle as a high-performance magnetic particle imaging tracer for stem cell tracking.</a> ACS Nano. 14 (2), 2053-2062 (2020).</li>
<li>Li, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Extravascular+sources+of+lung+angiotensin+peptide+synthesis+in+idiopathic+pulmonary+fibrosis%5BTitle%5D)+AND+%22Am+J+Physiol+Lung+Cell+Mol+Physiol%22%5BJournal%5D)">Extravascular sources of lung angiotensin peptide synthesis in idiopathic pulmonary fibrosis.</a> Am J Physiol Lung Cell Mol Physiol. 291 (5), L887-L895 (2006).</li>
</ol>

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<table><tbody><tr><td>1.7 mL microcentrifuge tube</td><td>DOT Scientific</td><td>RN1700-GMT</td><td>세포 수집 및 BLM 희석 준비용</td></tr><tr><td>Anti-Dextran 항체</td><td>STEMCELL Technologies</td><td>60026</td><td>일차 항체</td></tr><tr><td>BD Luer-Lok&nbsp;주사기</td><td>Becton, Dickinson</td><td>309628</td><td>기관 내 침착 중 공기 주입용</td></tr><tr><td>Cytospin 3</td><td>Thermo Shandon</td><td>74010121GB</td><td></td></tr><tr><td>DNase I</td><td>Millipore-Sigma</td><td>10104159001</td><td></td></tr><tr><td>Dulbecco's phosphate-buffered saline (DPBS -/-)&nbsp;</td><td>Gibco</td><td>14190250</td><td></td></tr><tr><td>Fetal Bovine Serum&nbsp;</td><td>ThermoFisher Scientific</td><td>A5670701</td><td></td></tr><tr><td>Gentamicin&nbsp;</td><td>Gibco</td><td>15750060</td><td></td></tr><tr><td>Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor&trade; 568</td><td>ThermoFisher Scientific</td><td>A-11004</td><td>이차 항체</td></tr><tr><td>Goat Serum</td><td>Millipore-Sigma</td><td>G9023</td><td></td></tr><tr><td>Isoflurane</td><td>Covetrus</td><td>11695067772</td><td>가스 마취</td></tr><tr><td>Isoflurane vaporizer</td><td>SOMNI Scientific</td><td>VS6002</td><td>마취 장치</td></tr><tr><td>Momentum</td><td>Magnetic Insight Inc</td><td></td><td>임상 전 자기 입자 이미징(MPI) 스캐너</td></tr><tr><td>Mouse intubation kit</td><td>OHAN</td><td>Ohan-201</td><td>모든 필수 액세서리가 포함된 완전한 삽관 키트</td></tr><tr><td>NOD/SCID Mice</td><td>Jackson Laboratory</td><td>RRID:IMSR_JAX:001303</td><td>면역 저하된 마우스 모델</td></tr><tr><td>Penicillin/Streptomycin&nbsp;</td><td>Gibco</td><td>15140122</td><td></td></tr><tr><td>PneumaCult-Ex Plus Medium&nbsp;</td><td>STEMCELL Technologies</td><td>5040</td><td></td></tr><tr><td>ProLong Diamond Antifade Mountant with DAPI&nbsp;</td><td>Invitrogen</td><td>P36962</td><td>면역 염색된 슬라이드용 장착 매체</td></tr><tr><td>Protease from Streptomyces griseus&nbsp;</td><td>Millipore-Sigma</td><td>P5147</td><td></td></tr><tr><td>Surgical kit</td><td></td><td></td><td>쥐의 혀를 잡는 데 사용되는 둔부 포스펫</td></tr><tr><td>Tris-Buffered Saline with Tween20 (TBST)</td><td>ThermoFisher Scientific</td><td>28360</td><td></td></tr><tr><td>TrypLE Express Enzyme&nbsp;</td><td>ThermoFisher Scientific</td><td>12604021</td><td></td></tr><tr><td>VivoTrax Plus</td><td>Magnetic Insight</td><td>MIVTP01</td><td>세포 표지에 사용되는 철 산화물 추적자 및 후속 MPI</td></tr></tbody></table>
```

자기 입자 이미징을 사용한 폐 섬유증에 대한 이식된 원위 폐 상피 전구 세포의 이미지 유도 생체 내 추적

Image-guided In Vivo Tracking of Transplanted Distal Lung Epithelial Progenitor Cells for Pulmonary Fibrosis Using Magnetic Particle Imaging

Pulmonary fibrosis (PF) is a progressive and chronic lung disease characterized by repeated alveolar epithelial injury that leads to excessive ...

image-guided-vivo-tracking-transplanted-distal-lung-epithelial

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작은 동물의 근육 Engraftment의 역학에 의해 추적 VIVO에서 형광 이미징

생체내 bioluminescence과 자기 공명 영상에서 (I), 그리고 (ii) 해부 histological 분석 :이 문서를 사용하여 쥐 두뇌에있는 세포 이식의 multimodal 이미징을위한 이벤트를 최적화된 순서를 설명합니다. 하나의 동물에서 이러한 이미징 modalities를 결합하면 높은 해상도, 민감도와 특이성과 세포 이식 평가를하실 수 있습니다.

마우스의 중추 신경계에서 줄기 세포 주입의 Multimodal 이미징

myoblast 이식의 성공 여부를 평가하는 비 침습적 방법이 설명되어 있습니다. 방법은 그의 표현 다른 이미징 modalities를 이미징 할 수 있습니다 유전자로 구성된 통합 된 퓨전 리포터 유전자의 장점을 걸립니다. 여기, 우리는...

이식 근육 전구 세포를 타겟팅 할 수 분자 이미징

MRI를 사용하여 세포 추적 지난 몇 년 동안 괄목할만한 주목을 받고있다. 이 프로토콜은 불소와 수지상 세포의 라벨 설명...

사용 모니터링 수지상 세포 이동<sup&gt; 19</sup&gt; F /<sup&gt; 1</sup&gt; H 자기 공명 영상

조혈 줄기 및 전구 세포의 표시 조합 5 형광 단백질은 재생 중에 골수 조혈 아키텍처에 대한 통찰력을 제공하는 공 초점과 두 광자 현미경을 통해 생체 클론 추적에 있습니다. 이 방법은 이식 다음 시간의 광범위한 기간에 대한 손상 조직의 스펙트럼으로 구분 HSPCs 유래 세포의 비 침습적 운명 매핑 할 수 있습니다.

공 초점 및 다중 광자 현미경을 사용하여 다섯 형광 단백질에 의해 표시됨 조혈 줄기 및 전구 세포의 생체 내 클론 추적에서

우리 비-침략 적 multimodal 접근 bleomycin의 이중 intratracheal 주입에 의해 유도 된 마우스 폐 섬유 증 모델의 경도 평가 대 한 마이크로-CT와 형광 분자 단층에 따라 이미지를 설명 합니다.

Multimodal 접근을 이미징 Bleomycin 유도 폐 섬유 증 생쥐에서의 경도 평가 대 한 마이크로-CT와 형광 분자 단층에 따라

이 기사에서는 H2052/484 중피종 세포를 면역 손상된 심질 마우스의 흉막 강내로 이식함으로써 인간 흉막 중피종의 정형외 마우스 모델의 생성을 설명합니다. 내림두근 종양의 발달에 대한 종방향 모니터링은 비침습적 다중모달[18F]-2-플루오로-2-디옥시-D-포도당 양면 방출 단층 촬영 및 컴퓨터 단층 촬영 영상에 의해 평가되었다.

심질 마우스에 있는 인간 흉막 중피종의 정형외 모형의 PET/CT에 의한 이식 및 모니터링

여기에 제시된 비침습적 중간엽 줄기 세포(MSC) 전달 및 외상성 뇌 손상의 마우스 모델에서 추적을 위한 프로토콜이 제시된다. 초파라마그네틱 산화물 나노입자는 실시간 MRI를 이용한 비강 내 전달 후 생체 내 추적 및 비침습적 MSC 라벨링을 위한 자기 공명 영상(MRI) 프로브로 사용된다.

외상성 뇌 손상 뮤린 모델에서 비강 내 전달 후 MRI를 사용하여 수퍼 파라마그네틱 철 산화물 표지 메젠키말 줄기 세포 추적

여기에서는 이광자 현미경을 사용하여 이식된 쥐의 왼쪽 폐를 생체 내 이미징하는 프로토콜을 제시합니다. 이는 쥐 폐 이식 후 실시간으로 세포 역학 및 상호 작용을 연구하는 데 유용한 도구입니다.

이식된 마우스 폐의 생체 내 2광자 현미경

이 연구는 형광 A549-iRFP 세포의 다세포 스페로이드의 폐 내 접종에 기반한 비소세포폐암(NSCLC) 모델을 제시합니다. 이 모델은 임상 비소세포폐암(NSCLC) 단계를 요약하고 시스플라틴(cisplatin)에 반응하며, 이는 장파장 형광의 동적 생체 내 모니터링에 따른 것입니다.

비소세포폐암의 기형외형 이종이식 모델 구축: 질병 진행 모방 및 약물 활성성 예측

배우세요 자기 입자 이미징을 사용하여 폐 섬유증 모델에서 이식된 원위 폐 상피 전구 세포를 비침습적으로 실시간 추적하는 방법을. 이 기술은 세포 치료의 분포와 유지를 정량적으로 평가하여 재생 의학 연구를 지원합니다.

자기 입자 이미징을 사용한 폐 섬유증에 대한 이식된 원위 폐 상피 전구 세포의 이미지 유도 생체 내 추적

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