Name: 경추 림프관 캐뉴럴 쥐 모델을 이용한 머리와 뇌로부터의 림프 수송 평가 (동영상) | JoVE Journal
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Description: 배우세요 경추 림프관 캐뉴럴 기법을 이용하여 쥐에서 뇌와 머리로부터 림프 수송을 정량적으로 측정하는 방법을. 이 프로토콜은 중추신경계 림프 배출 및 약물 제거 연구에 활용됩니다.

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<li>de Jong, W. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Long-term+cannulation+of+the+vena+cava+of+rats+for+blood+sampling%3A+local+and+systemic+effects+observed+by+histopathology+after+six+weeks+of+cannulation%5BTitle%5D)+AND+%22Lab+Anim%22%5BJournal%5D)">Long-term cannulation of the vena cava of rats for blood sampling: local and systemic effects observed by histopathology after six weeks of cannulation.</a> Lab Anim. 35 (3), 243-248 (2001).</li>
</ol>

<table><tbody><tr><td>30게이지 바늘</td><td>유쇼 헬스</td><td></td><td>카뉼라의 근위 끝(0503)과 헤파린 주사기를 연결하는 데 사용됩니다</td></tr><tr><td>증기화기가 포함된 마취 디스펜서</td><td>윤 기기 회사</td><td></td><td></td></tr><tr><td>둔탁한 겸자</td><td>RWD 생명과학</td><td>F22002-10&nbsp;</td><td></td></tr><tr><td>면봉</td><td>칭다오 슐츠 바이오테크놀로지</td><td>MQ-02</td><td>근육 조직을 일시적으로 고정하는 데 사용되었습니다</td></tr><tr><td>가열 수술용 패드</td><td>상하이 이헝 인스트루먼트</td><td></td><td>차원 15 &; 20cm</td></tr><tr><td>헤파린 나트륨</td><td>상하이 알라딘 생화학 기술</td><td>H123383</td><td>사용 전 희석 필요</td></tr><tr><td>인슐린 주사기 (1 mL)</td><td>유쇼 헬스</td><td>U100-1ML</td><td>헤파린 프리 코팅에만 사용됩니다</td></tr><tr><td>흡입용 이소플루란 용액</td><td>RWD 생명과학</td><td>R510-22-10</td><td>증기화를 통한 마취에 사용됨</td></tr><tr><td>마커 펜</td><td>어떤 브랜드든</td><td>해당 없음</td><td></td></tr><tr><td>마이크로 원심분리기 튜브 (1.5 mL)</td><td>난통 하이루이 실험 장비</td><td>HR10003</td><td>림프 수집에 사용</td></tr><tr><td>마이크로 가위</td><td>RWD 생명과학</td><td>GD234BA0007925</td><td></td></tr><tr><td>나일론 수술용 봉합 및 nbsp;</td><td>상하이 이헝 인스트루먼트</td><td>4-0 35CM</td><td></td></tr><tr><td>피펫(용량 조절 가능, 측정 범위 5에서 50 & mu; L)</td><td>드래곤 연구소 기기 주식회사</td><td>7030301006</td><td>조직 접착제 측정에만 사용됩니다</td></tr><tr><td>피펫 팁 &nbsp;</td><td>바이오샤프 생명과학</td><td>BS-10-T</td><td>조직 접착 전이에만 사용되며, 특정 부피 요구 사항 없음</td></tr><tr><td>폴리에일렌(PE) 캐뉼라 0.5mm 외경, 0.3mm 내경 (0503)</td><td>애니랩 과학 기기(닝보)</td><td>PE-0503</td><td></td></tr><tr><td>폴리에일렌(PE) 캐뉼라 0.6mm 외경, 0.5mm 내경 (0605)</td><td>애니랩 과학 기기(닝보)</td><td>PE-0605</td><td></td></tr><tr><td>면도날</td><td>질레트 상하이</td><td>Q31/0115000301C004</td><td>털 면도에만 사용됩니다</td></tr><tr><td>고무줄</td><td>어떤 브랜드든</td><td>해당 없음</td><td>쥐의 앞다리를 제압하는 데 사용됨</td></tr><tr><td>통치자</td><td>어떤 브랜드든</td><td>해당 없음</td><td></td></tr><tr><td>스케이플 블레이드 (#23)</td><td>RWD 생명과학</td><td>S31010-01</td><td></td></tr><tr><td>염화나트륨(0.9%) 주입용 용액</td><td>스자좡 제4 제약</td><td>H20066533 (승인 번호)</td><td>털 헹구기에만 사용됨</td></tr><tr><td>스프래그-도울리 래츠</td><td>베이징 바이탈 리버 실험 동물 기술</td><td>101</td><td>무게 220에서 250그램, 나이 제한 없음</td></tr><tr><td>수술용 조명</td><td>베이징 허 바이오테크</td><td>XHA011-1</td><td></td></tr><tr><td>외과용 현미경</td><td>상하이 체웨이 광전자 기술</td><td>SMZ755T</td><td>기계 스테이지가 없는 해부 현미경은 수납식 스탠드를 장착할 수 있습니다</td></tr><tr><td>조직 접착제</td><td>3M 베트본드</td><td>1469SB</td><td>주성분은 시아노아크릴레이트와 nbsp;</td></tr><tr><td>조직 확장기 (Colibri)</td><td>RWD 생명과학</td><td>R2029-04</td><td>확장 범위 20mm/4cm</td></tr></tbody></table>

경추 림프관 캐뉴럴 쥐 모델을 이용한 머리와 뇌로부터의 림프 수송 평가

Cervical Lymph Duct-Cannulated Rat Model for Assessing Lymphatic Transport from the Head and Brain

Lymph flows from the central nervous system (CNS) via a series of lymphatic vessels (LVs) and lymph nodes that converge at the cervical lymph ducts. In ...

cervical-lymph-duct-cannulated-rat-model-for-assessing-lymphatic

Research

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JoVE Journal

Immunology and Infection

면역학 및 감염학

이 비디오는 쥐의 장간막 림프관을 캐뉼레이션하는 기술을 보여줍니다. 이 방법을 사용하면 림프액에 존재하는 전체 성분에 직접 접근할 수 있습니다.

Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest 림프액을 수확합니다.

이 비디오는 쥐 모델에서 십이지장 내 삽관을 보여줍니다. 쥐를 마취 한 후 십이지장을 찾고 작은 구멍을 만듭니다. 끝이 비스듬한 J자형 캐뉼라를 조심스럽게 삽입하고 고정한 다음 절개 부위를 닫습니다. 캐뉼러형 쥐 모델은 면역병리학 연구를 위한 장 내 주입을 가능하게 합니다.

면역병리학 연구를 위한 쥐 모델의 십이지장 내 삽관(intraduodenal cannulation)

이 비디오에서는 쥐 모델에서 왼쪽 경동맥을 캐뉼링하는 수술 절차를 보여줍니다.

쥐 모델에서 경동맥의 캐뉼레이션(cannulation of the carotid artery)

여기에서는 쥐의 장간막 림프관을 캐뉼로화하는 기술을 설명하며, 이를 통해 장 전달 후 림프계를 통한 지질 및 약물 수송을 정량화할 수 있습니다. 이 기술은 장간막 림프 농도 및/또는 유체, 면역 세포, 펩타이드, 단백질 및 친유성 분자의 수송을 평가하는 데 적용할 수 있습니다.

장간막 림프 덕트 유관 쥐 모델 : 창자 림프 약물 운송의 평가에 응용

여기는 시스테 마그나 cannulation (CMc), 뇌 척추 액체 (CSF)에 추적기, 기판 및 신호 분자를 제공 하는 최소 침 습 방법을 수행 하는 프로토콜에 설명 합니다. 다른 이미지 형식으로 결합, CMc glymphatic 시스템 및 CSF 역학 평가 뿐만 아니라 다양 한 화합물의 두뇌 전체 배달 수 있습니다.

설치류의 시스테 마그나에 정 맥 주입

여기서는 흉부 덕트 림프구를 수집하고 시간 경과 사진을 사용하여 3 시간 동안 쥐 페이어의 패치에서 장 열대 림프구의 이동을 관찰하는 정확한 방법을 설명합니다. 이 기술은 림프구의 역학이 염증 성 조건하에서 어떻게 영향을 받는지 명확히 할 수 있습니다.

쥐 페이어의 패치에서 림프구 역학의 현미경 관찰

여기에서는 다른 변수의 영향을 최소화하고 수술 기반 연구 수행을 표준화하기 위해 쥐의 심부 경부 림프절 또는 구심성 림프관을 안면 후방 정맥과 문합하는 단계를 명확하게 정의하는 프로토콜을 제시합니다.

쥐 모델에서 심부 경추 림프정맥 문합을 위한 수술 프로토콜: 림프절 및 림프관 문합

이 실험 프로토콜은 간 림프관 캐뉼 쥐 모델을 설명하여 간 림프 및 혈관 배출을 직접적이고 정량적으로 평가할 수 있게 합니다. 이 모델은 간림프와 전신 혈액을 동시에 샘플링하여 간 수송, 대사, 면역 신호 전달을 조사할 수 있게 하며, 약물 약물동학과 간 특이적 생물학 연구를 지원합니다.

림프와 혈관 동시 샘플링을 통한 간 수송 경로를 평가하는 포괄적인 쥐 모델

사타구니 림프절 (LN)의 intravital 현미경을 수행하기위한 기법이 설명되어 있습니다. 이러한 기술은 실시간으로...

사타구니 림프 노드의 Intravital 현미경

2 - 광자 현미경의 최근 발전은 실시간으로...

마우스 오금 림프절의 Intravital 이미징

배우세요 경추 림프관 캐뉴럴 기법을 이용하여 쥐에서 뇌와 머리로부터 림프 수송을 정량적으로 측정하는 방법을. 이 프로토콜은 중추신경계 림프 배출 및 약물 제거 연구에 활용됩니다.

경추 림프관 캐뉴럴 쥐 모델을 이용한 머리와 뇌로부터의 림프 수송 평가

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