Name: 자가 조립 인간 심장 세포에서의 저산소증-재산소화 손상 모델 (동영상) | JoVE Journal
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Description: 배우세요 iPSC 유래 자가 조립 심장 오가노이드에서 저산소증-재산소화 손상을 모델링하는 방법을. 이 프로토콜은 심근경색 유사 손상을 재현하고 재생 치료법을 테스트하는 인간 특이적 플랫폼을 제공합니다.

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<li>Lai, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Reciprocal+analyses+in+zebrafish+and+medaka+reveal+that+harnessing+the+immune+response+promotes+cardiac+regeneration%5BTitle%5D)+AND+%22Elife%22%5BJournal%5D)">Reciprocal analyses in zebrafish and medaka reveal that harnessing the immune response promotes cardiac regeneration.</a> Elife. 6, e25605(2017).</li>
</ol>

<table><tbody><tr><td>아큐타제 용액</td><td>시그마-올드리치</td><td>A6964-500ML</td><td></td></tr><tr><td>항심장 트로포닌 T 항체</td><td>앱캠</td><td>EPR20266</td><td></td></tr><tr><td>B-27 보충제(50X), 혈청 무첨가</td><td>깁코</td><td>17504044</td><td></td></tr><tr><td>인슐린 제외 B-27 보충제</td><td>깁코</td><td>A1895602</td><td></td></tr><tr><td>바이오텍 시너지 네오2 플레이트 리더기</td><td>바이오텍</td><td></td><td></td></tr><tr><td>캘리포니아-520, AM</td><td>AAT 바이오퀘스트</td><td>21131</td><td></td></tr><tr><td>카디오카운트 V1</td><td>카라 연구소</td><td>PMID: 38984052</td><td></td></tr><tr><td>세포주 (Homo sapiens, 남성) - DU11 iPSC 계통</td><td>듀크 대학교 줄기세포 코어</td><td></td><td></td></tr><tr><td>충전 현미경 슬라이드, 흰색 유리</td><td>VWR</td><td>89500-496</td><td></td></tr><tr><td>CHIR 99021</td><td>토크리스</td><td>4423</td><td></td></tr><tr><td>클론R</td><td>스템셀 테크놀로지</td><td>5888</td><td></td></tr><tr><td>코닝 96-웰 클리어 초저 부착 마이크로플레이트</td><td>피셔</td><td>7007</td><td></td></tr><tr><td>일회용 피펫팅 저장소</td><td>VWR</td><td>89094-678</td><td></td></tr><tr><td>DMEM/F-12, HEPES</td><td>깁코</td><td>11330057</td><td></td></tr><tr><td>EVOS 형광 현미경</td><td>EVOS</td><td></td><td></td></tr><tr><td>겔트렉스 저축성장인자 저막 매트릭스, LDEV 무첨가, 줄기세포 인증</td><td>깁코</td><td>A1413302</td><td></td></tr><tr><td>인간 활성인 재조합 단백질</td><td>페프로텍 주식회사/라이프 테크놀로지</td><td>120-14E-50UG</td><td></td></tr><tr><td>IWP-2</td><td>스템셀 테크놀로지</td><td>72124</td><td></td></tr><tr><td>L-아스코르브산 (결정성/인증된 ACS)</td><td>피셔 사이언티브</td><td>A61-100</td><td></td></tr><tr><td>마우스 항비멘틴</td><td>DSHB</td><td>AMF-17b</td><td></td></tr><tr><td>mTeSR 플러스</td><td>스템셀 테크놀로지</td><td>100-0276</td><td></td></tr><tr><td>멀티웰 세포 배양 플레이트 & ndash; 12 우물</td><td>VWR</td><td>10062-894</td><td></td></tr><tr><td>플루로닉 F-127</td><td>시그마-올드리치</td><td>P2443</td><td></td></tr><tr><td>릴레SR</td><td>스템셀 테크놀로지</td><td>100-0483</td><td></td></tr><tr><td>RPMI 1640 미디엄</td><td>깁코</td><td>11875119</td><td></td></tr><tr><td>RPMI 1640 미디엄, 페놀 레드 없음</td><td>깁코</td><td>11835-030</td><td></td></tr><tr><td>티아조비빈</td><td>스템셀 테크놀로지</td><td>72254</td><td></td></tr><tr><td>티슈텍 O.C.T. 복합물</td><td>사쿠라 파인텍 미국 주식회사 (VWR)</td><td>25608-930</td><td></td></tr><tr><td>자이스 회전 디스크 공초점 현미경</td><td>자이스</td><td></td><td></td></tr></tbody></table>

자가 조립 인간 심장 세포에서의 저산소증-재산소화 손상 모델

A Hypoxia-Reoxygenation Injury Model in Self-Assembling Human Cardioids

Without rapid revascularization, ischemic heart disease leads to irreversible loss of cardiomyocytes, scar formation, and decreased cardiac function. The ...

a-hypoxia-reoxygenation-injury-model-self-assembling-human

Research

연구

JoVE Journal

Medicine

의학

이 원고는 표면 마커 발현과 세포 분류를 사용하여 정의된 공학적 심장 조직의 생성을 설명합니다. 그런 다음 정의된 조직을 다중 조직 생물반응기에서 사용하여 심장 세포 치료의 메커니즘을 조사하여 기능적이면서도 제어된 인간 심장 모델 시스템을 제공할 수 있습니다.

정의 인간 공학 심장 조직의 건설 심장 세포 치료의 메커니즘을 연구하는

여기, 선물이 수혈 또는 쥐 inotropic 에이전트 없이 단순 복구 심폐 바이패스 모델을 설명 하는 프로토콜. 이 모델의 장기 연구 심폐 바이패스의 여러 기관 sequelae 수 있습니다.

수혈 또는 쥐 Inotropic 에이전트 없이 복구 심폐 바이패스 모델

인간 유도만능 줄기세포로부터 유래된 심근세포를 이용한 허혈성 심장질환 모델을 제시하고, 허혈에 의한 조직 손상의 정량적 평가를 위한 방법을 제시한다. 이 모델은 약물 검진및 허혈성 심장 질환에 대한 추가 연구를위한 유용한 플랫폼을 제공 할 수 있습니다.

허혈성 심장 질환 및 심장 근막 수축의 비디오 기반 비교 모델

심근 보호에 저체온증의 다른 정도의 뚜렷한 영향은 철저하게 평가되지 않았습니다. 본 연구의 목표는 인간 심근세포 기반 모델에서 다른 저체온증 치료에 따른 세포 사멸 의 수준을 정량화하여 미래의 심층 분자 연구의 토대를 마련하는 것이었습니다.

인간 심장 근세포 모델에서 저체온증-사전 조절 에 따른 심근 보호의 체외 평가

이 프로토콜의 목표는 심장 조직 공학, 약물 스크리닝 및 질병 모델링을 위해 심장 섬유아세포(CFs)와 공동 배양된 줄기 세포 유래 심근세포(CFs)로 구성된 고도로 정렬된 인간 심장 조직의 3차원(3D) 미세유체 모델의 개발을 설명하고 시연하는 것이다.

미세 유체 플랫폼 내에서 3D 조직 된 인간 심장 조직 개발

공간 거리는 별도의 내피 및 심근 세포 세포층의 공동 배양 모델에서 저산소증 / 재 산소화 손상을 평가하는 핵심 매개 변수이며, 처음으로 공동 배양 공간 환경을 최적화하여 심근 세포 보호에서 내피 세포의 역할을 테스트하기위한 유리한 시험관 내 모델을 제공 할 필요가 있음을 시사합니다.

체외에서 심장 허혈/재관류를 모방하기 위한 세포 공동 배양 모델 개발

여기서, 우리는 자기 조직에 의하여 인간 다능성 줄기 세포를 사용하여 발달적으로 관련있는 인간 심장 오르가노이드 (hHOs)를 만드는 프로토콜을 기술합니다. 이 프로토콜은 발달 단서의 순차적 활성화에 의존하고 매우 복잡하고 기능적으로 관련된 인간 심장 조직을 생성합니다.

다능성 줄기 세포에서 파생된 자가 조립 인간 심장 오르가노이드 생성

줄기 세포 유래 심근 세포를 사용하여 생체 공학적으로 설계된 3차원 심장 조직은 건강한 심근과 병든 인간 심근 을 체외 에서 연구하는 동시에 천연 심장 틈새 시장의 주요 측면을 요약하기 위한 유망한 모델로 부상했습니다. 이 원고는 인간 유도 만능 줄기 세포 유래 심근세포에서 생성된 high-content engineered 심장 조직을 제작하고 분석하기 위한 프로토콜을 설명합니다.

공학적 심장 조직의 데이터 수집 및 모델 처리량을 개선하기 위한 바이오리액터 설계

여기서는 고대사성 근위 소관 상피 세포에서 저산소증/산소 재산화 손상을 모델링하는 시험관 내 프로토콜을 설명합니다. 이 모델은 대사 스트레스 유발 손상을 유도하도록 설계되었으며, 근위 세뇨관 손상 지표의 발현과 미토콘드리아 호흡 기능 평가를 통해 측정할 수 있습니다.

근위 관 상피 세포에서 저산소증/재산소화 손상 모델링

심각한 척수 손상의 타박상 모델이 설명되어 있습니다. 자세한 수술 전, 수술 및 수술 후 단계는 일관된 모델을 얻기 위해 설명되어 있습니다.

쥐에 심각한 척수 손상의 타박상 모델

배우세요 iPSC 유래 자가 조립 심장 오가노이드에서 저산소증-재산소화 손상을 모델링하는 방법을. 이 프로토콜은 심근경색 유사 손상을 재현하고 재생 치료법을 테스트하는 인간 특이적 플랫폼을 제공합니다.

자가 조립 인간 심장 세포에서의 저산소증-재산소화 손상 모델

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