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maintenance

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enableNewMyJoveSectionDesign

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freeAccessWindow

ssoRoleCapture

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disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol>
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</ol>

```html
<table>
	<tbody>
		<tr>
			<td>10% Tween-20</td>
			<td>Bio-Rad Laboratories</td>
			<td>1662404</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>20x PBS, pH7.4</td>
			<td>Growcells.com</td>
			<td>MRGF-6396</td>
			<td>2.74M NaCl, 53.7 mM KCl, 20.3 mM Na2HPO4, 29.4 mM KH2PO4&nbsp;</td>
		</tr>
		<tr>
			<td>20x SSC</td>
			<td>Quality Biological</td>
			<td>351-003-131</td>
			<td>3M NaCl, 0.3M Sodium Citrate</td>
		</tr>
		<tr>
			<td>37 &deg;C incubator</td>
			<td>Fisher Scientific (Isotemp)</td>
			<td>15-015-2633</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>37 &deg;C water bath</td>
			<td>Fisher Scientific</td>
			<td>FSGPD15D</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Aluminum foil</td>
			<td>Reynolds Wrap/Fisher Brand</td>
			<td>15-078-291</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Antibody: Mouse-anti-Abrupt</td>
			<td>DSHB</td>
			<td>Abrupt; RRID: AB_528344&nbsp;</td>
			<td>Dilution: 1:20</td>
		</tr>
		<tr>
			<td>Antibody: Chicken anti-GFP</td>
			<td>Millipore Sigma</td>
			<td>Catalog# 06-896; RRID: AB-619712</td>
			<td>Dilution: 1:500</td>
		</tr>
		<tr>
			<td>Antibody: Rabbit anti-GFP</td>
			<td>Life Technologies</td>
			<td>#11122; RRID: AB_221569</td>
			<td>Dilution: 1:500</td>
		</tr>
		<tr>
			<td>Antibody: Rabbit anti-Dll</td>
			<td>Genscript/Desplan lab</td>
			<td>-</td>
			<td>Dilution: 1:200</td>
		</tr>
		<tr>
			<td>Antibody: Rat anti-NCadherin</td>
			<td>DSHB</td>
			<td>DN-Ex #8, RRID: AB_528121</td>
			<td>Dilution: 1:50</td>
		</tr>
		<tr>
			<td>Antibody: Rabbit anti-SoxN</td>
			<td>Genscript/Desplan lab</td>
			<td>-</td>
			<td>Dilution: 1:200</td>
		</tr>
		<tr>
			<td>Antibody: Guinea Pig anti-Traffic jam</td>
			<td>Gift from D. Godt</td>
			<td>-</td>
			<td>Dilution: 1:2,000</td>
		</tr>
		<tr>
			<td>Antibody: Donkey anti-Rat 405</td>
			<td>Jackson ImmunoResearch</td>
			<td>Code # 712-475-150; RRID: AB_2340680</td>
			<td>Dilution: 1:100</td>
		</tr>
		<tr>
			<td>Antibody: Donkey anti-Chicken 488</td>
			<td>Jackson ImmunoResearch</td>
			<td>Code #703-545-155; RRID: AB_2340375</td>
			<td>Dilution: 1:200</td>
		</tr>
		<tr>
			<td>Antibody: Donkey anti-Mouse 488</td>
			<td>Jackson ImmunoResearch</td>
			<td>Code #715-545-151; RRID: AB_2341099</td>
			<td>Dilution: 1:200</td>
		</tr>
		<tr>
			<td>Antibody: Donkey anti-Rabbit 488</td>
			<td>Jackson ImmunoResearch</td>
			<td>Code #711-545-152; RRID: AB_2313584</td>
			<td>Dilution: 1:200</td>
		</tr>
		<tr>
			<td>Antibody: Donkey anti-Rabbit A555</td>
			<td>Thermo Scientific</td>
			<td>Catalog #A-31572; RRID: AB_162543</td>
			<td>Dilution: 1:200</td>
		</tr>
		<tr>
			<td>Antibody: Donkey anti-Rat Cy3</td>
			<td>Jackson ImmunoResearch</td>
			<td>Code #712-165-153; RRID: AB_2340667</td>
			<td>Dilution: 1:200</td>
		</tr>
		<tr>
			<td>Antibody: Donkey anti-Guinea Pig 647</td>
			<td>Jackson ImmunoResearch</td>
			<td>Code #706-605-148; RRID: AB_2340476</td>
			<td>Dilution: 1:200</td>
		</tr>
		<tr>
			<td>Antibody: Donkey anti-Rat 647</td>
			<td>Jackson ImmunoResearch</td>
			<td>Code #712-605-153; RRID: AB_2340694</td>
			<td>Dilution: 1:200</td>
		</tr>
		<tr>
			<td>Antifade mountant</td>
			<td>Thermo Fisher Scientific</td>
			<td>S36940 (SlowFade Gold Antifade Mountant)</td>
			<td>Other slowfades will work as well, including ones with DAPI (S36936)</td>
		</tr>
		<tr>
			<td>Confocal Microscope</td>
			<td>Zeiss</td>
			<td>LSM-880</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Cover slips (22 x 30)</td>
			<td>VWR</td>
			<td>16004-332</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Fly: D. melanogaster: ab-GFP-FLAG</td>
			<td>BDSC</td>
			<td>38626; RRID: BDSC_38626</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Fly: D. melanogaster: Canton S</td>
			<td>BDSC</td>
			<td>64349; RRID: BDSC_64349</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Fly: D. melanogaster: dpp-Gal4</td>
			<td>BDSC</td>
			<td>1553; RRID: BDSC_1553</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Fly: D. melanogaster: ham-GFP.FPTB</td>
			<td>BDSC</td>
			<td>83660; RRID: BDSC_83660</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Fly: D. melanogaster: yw; 10xUAS-myr-GFP;</td>
			<td>BDSC</td>
			<td>32198; RRID: BDSC_32198</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Fly: D. melanogaster: UAS-Stinger</td>
			<td>BDSC</td>
			<td>84277; RRID: BDSC_84277</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Fly: D. melanogaster:&nbsp; wit-KO-Gal4</td>
			<td>BDSC</td>
			<td>600136; RRID: BDSC_600136</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Forceps</td>
			<td>Dumont</td>
			<td>#5 or #55s</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Glass slides</td>
			<td>VWR</td>
			<td>16005-106</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>Glycine</td>
			<td>Millipore Sigma</td>
			<td>G7126-10MG</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>HCR Amplification buffer</td>
			<td>Molecular Instruments</td>
			<td>HCR Gold RNA-FISH Kit</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>HCR-Gold Amplifiers</td>
			<td>Molecular Instruments</td>
			<td>HCR Gold RNA-FISH Kit</td>
			<td>NA</td>
		</tr>
		<tr>
			<td>HCR HiFi Probes</td>
			<td>Molecular Instruments</td>

발달 중인 초파리 시엽에서의 잡종화 연쇄 반응 fish

Hybridization Chain Reaction FISH in the Developing Drosophila Optic Lobe

발달 중인 초파리 시엽에서의 잡종화 연쇄 반응 FISH

Understanding neural development requires precise characterization of when and where genes are expressed. Techniques such as in situ hybridization have ...

hybridization-chain-reaction-fish-developing-drosophila-optic

Research

연구

JoVE Journal

Neuroscience

신경과학

전체 마운트를 사용하여 현장에서</em분자 생물학과 Organismal를 링크> 하이브 리다이 제이션

의 형태학의 분석 Drosophila</em유전 모자이크 사용하기> 애벌레 주변 감각 신경 세포의 Dendrites 및 Axons

여기 우리는 전체 마운트 형광을...

전체 마운트 RNA 형광<em&gt; 현장에서</em의&gt; 하이브리드<em&gt; Drosophila</em&gt; 태아

제브라 피쉬, 작은 열대어, 척추 발달과 질병 중 유전자 기능 연구를위한 대중적인 모델이되고있다. 표적 유전자의 시간적, 공간적 표현에 의해 결정될...

높은 해상도 전체 마운트<em&gt; 제자리에서</em유전자 발현 및 기능을 연구하는 제브라 피쉬 배아에서&gt; 하이브리드

면역염색은 조직 내의 특정 세포 유형과 단백질을 시각화하는 효과적인 기술입니다. 초음파 처리를 활용함으로써, 여기에 설명된 프로토콜은 면역 염색 전에 후기 단계의 배아와 유충에서 Drosophila melanogaster 조직을 해부해야 할 필요성을 완화합니다. 우리는 포름알데히드가 고정된 전체 산 유충의 면역 염색을 위한 효율적인 방법론을 제공합니다.

늦게 단계 배아와 애벌레의 초음파 - 촉진 면역 형광 염색<em&gt; 초파리</em&gt; 조직<em&gt; 제자리에서</em

우리는 색의 침전물을 대조하여 세 가지 다른 mRNA의 분포 패턴의 동시 특정 검출을 허용 그대로 초파리 배아에서 멀티 타겟 발색 전체 마운트 현장 하이브리드에 (MC-WISH) 절차를 설명합니다.

멀티 타겟 원체 전체 마운트<em&gt; 현장에서</em&gt; 하이브리드에서 유전자 발현의 도메인을 비교<em&gt; 초파리</em&gt; 배아

교 잡 프로토콜 설명된 RNA 제자리에 전체 초파리 배아 또는 해 부 조직에서 RNA의 검출을 허용 한다. 96-잘 결정 접시와 tyramide 신호 증폭에 사용 하 여, 성적 감지할 수 있습니다 높은 해상도, 감도, 및 처리량, 그리고 상대적으로 저렴 한 비용.

높은 해상도 교 잡 형광 제자리에 초파리 태아와 Tyramide 신호 증폭을 사용 하 여 조직에서

이 원고 X를 생성 하는 자세한 방법을 제공-염색체 팔 프로브 및 수행 형광 제자리에서 교 잡 (물고기) 자매의 상태를 검사 하 chromatid 응집력 prometaphase 및 를 체포 하는 분열 초파리 oocytes. 이 프로토콜은 meiotic 팔 단결력은 그대로 또는 다른 genotypes에 방해 여부 결정 하는 데 적합 합니다.

Prometaphase 팔 응집력과 분열의 상태를 모니터링 하려면 형광 제자리에서 교 잡 (물고기)를 사용 하 여 나 초파리 Oocytes

이 기사에서는 모기 안테나와 상악 팔프에서 화학 감각 수용체 유전자의 공간 및 세포 분해능에 대한 통찰력을 밝히기 위해 혼성화 연쇄 반응 RNA 전체 장착 형광 in situ 교잡(HCR, RNA WM-FISH)을 수행하는 데 필요한 방법과 시약을 설명합니다.

혼성화(hybridization) 연쇄반응 RNA Whole-Mount Fluorescence In situ Hybridization of Chemosensory Genes in Mosquito Olfactory Appendages

Expansion-Assisted Iterescence In Situ Hybridization(EASI-FISH)은 확장 현미경 검사와 FISH(Fluorescence in situ hybridization)를 결합하여 두꺼운 조직에서 여러 유전자의 발현을 검출하는 강력한 기술입니다. 이 프로토콜은 이 방법의 최근 발전과 온전한 초파리 중추 신경계를 라벨링하기 위한 적응을 간략하게 설명합니다.

Expansion-Assisted Iterescence In Situ Hybridization을 사용한 온전한 초파리 뇌의 유전자 발현의 다중 검출

New York University.  이 프로토콜은 초파리 흑개척 유충 시립엽에서 하이브리드화 연쇄 반응 형광 제자리 하이브리다이제이션(HCR-FISH)을 수행하는 데 필요한 단계를 설명합니다. 탐침 설계, 하이브리다이제이션, 증폭, 조직 장착 절차가 상세히 설명되어 발달 중인 시엽의 시각화를 용이하게 합니다.

발달 중인 초파리 시엽에서의 잡종화 연쇄 반응 FISH

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