Spatial navigation and decision-making are involved processes, modulated by motivational drive, reward expectation, and working memory. Due to this complexity, many questions remain as to the cellular and circuit-level mechanisms that contribute to spatial processing. Technological advances (such as in electrophysiology, imaging techniques, and genetic manipulations) have greatly increased the complexity of questions related to spatial navigation that can be asked and answered. These advances also require careful, simultaneous consideration of appropriate behavioral tasks and environmental perturbations. The aim of this collection is to gather a diverse collection of methods, spanning from molecular to behavioral, that can be used to inspire lines of inquiry into the brain mechanism involved in spatial navigation. Ideally, this collection will lead to increases in our knowledge of how spatial information is processed in the brain.