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TOPICAL COLLECTIONS

Creative Methods for the Visualization of Morphogenic Dynamics

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Guest Editor

Jin Man Kim

Jin Man Kim

Seoul National University, School of Dentistry

<p>Dr. Jin Man Kim is an assistant professor at the School of Dentistry, Seoul National University. He completed an M.D.-Ph.D. course in 2015, majoring in cell biology. One of the major goals of his research is to achieve a comprehensive understanding of the signaling network mediating the epithelial-mesenchymal interaction during the development of epithelial organs. His work focuses on signaling dynamics from the single-cell (microscopic) to the tissue (mesoscopic) scale during developmental process. To monitor signaling dynamics at a single cell and a subcellular level, he utilizes the fluorescent protein-based genetically encoded biosensors the are customized to the specific biological context. In addition to a diverse range of cell lines, diverse types of organ explant cultures from mouse embryos, including those from the lungs, mammary glands, salivary glands, and tooth germ, are frequently used in his research. Using these strategies, he investigates the spatiotemporal effects of a diverse range of signaling messengers on the cytoskeleton and related physical events. Recently, he applied next-generation sequencing technology to determine the genetic background underlying the observed phenomena.</p>

Collection Overview

Morphogenesis is a complex event that establishes the correct tissue architecture. It is crucial for the specific functions of developed tissues. Investigations of spatiotemporal changes in intra- and extracellular activities have provided key clues fora comprehensive understanding of the principles of the morphogenic process. To monitor these activities, visualization methods that use current optical technologies have been extremely useful to expand our knowledge in the field of developmental biology. To achieve the successful execution of these techniques, integrated optical and biological expertise is required. In addition to the selection of the appropriate optical tools (e.g., type of microscope), it is also critical to design an adequate optical readout that reflects the biological activity of the imaging targets.

This collection session welcomes state-of-the-art, creative technologies to visualize biophysical as well as biochemical dynamics during morphogenesis. The imaging targets include various developing biological systems, including those at the subcellular, tissue, and whole-organ scale. Examples of methods that may be included in this collection are: original imaging processes designed for specific tissues or organs, improvements in or novel designs of biosensors and chemical dyes, optical technologies (e.g., enhanced spatiotemporal resolution), improved culture conditions for visualization (e.g., increasing cell permeability), and intravital imaging techniques.