Oxidative stress and immune responses are increasingly recognized as interconnected drivers of acute and chronic liver injury, influencing disease initiation, progression, tissue repair, and therapeutic outcomes across a broad spectrum of liver disorders. Despite significant advances in redox biology, immunology, and molecular technologies, experimental approaches for investigating oxidative stress– immune crosstalk remain diverse and often lack methodological standardization, limiting reproducibility and cross-study comparisons.

This Topical Collection aims to provide a comprehensive resource of high-quality visual protocols showcasing state-of-the-art experimental models and methodologies for studying oxidative stress– immune interactions in liver injury. Areas of interest include in vitro and in vivo liver injury models, reactive oxygen species detection, immune cell isolation and functional characterization, mitochondrial biology, immunometabolism, ferroptosis and other regulated cell death pathways, advanced imaging, organoid systems, and multi-omics technologies.

By bringing together reproducible protocols from leading investigators, this collection will promote methodological standardization, facilitate interdisciplinary collaboration, and accelerate mechanistic and translational research in hepatology, redox biology, and immunology. It will serve as a practical reference for researchers seeking reliable experimental techniques to advance our understanding of liver disease and support the development of innovative therapeutic strategies.