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Environmental Sciences

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Environmental Sciences

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Environmental Microbiology

RT-PCR for Detecting RNA in Samples

Description

RT-PCR is used to detect RNA in environmental samples by first converting RNA into DNA. It follows the same temperature-cycling steps as conventional PCR, but it begins with ribonucleic acid (RNA) instead of deoxyribonucleic acid (DNA). The RNA is turned into complementary DNA, or cDNA, so DNA-based tools can be used to stud...

Transcript

Reverse Transcriptie Polymerase Kettingreactie, of RT-PCR, maakt de detectie van RNA-virussen en microbiële genexpressie in het milieu mogelijk.

In tegenstelling tot cellulaire organismen van mensen tot bacteriën, die DNA als hun genetisch materiaal gebruiken, hebben sommige virussen genomen die uit RNA bestaan, inclusief veel pathogene vi...

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