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HIGH SCHOOL

Environmental Sciences

Science Experiments

Environmental Sciences

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Environmental Microbiology

Visualizing Environmental Bacteria with Gram Stain

Description

Gram staining helps scientists visualize bacteria collected from environmental sources. It is a fast, practical method for sorting culturable bacterial populations of interest. For environmental microbiologists, this can be useful whether the sample comes from a known isolate in the lab or from soil or water collected in the...

Transcript

Gram staining allows for quick visualization of bacterial morphology and broad cellular distinction from a wide range of environmental samples. To stain bacteria, a uniform smear is applied to a glass side and allowed to dry. After heat-fixing the smear, crystal violet is applied.

A decolorizing agent rinses away the crystal violet from Gr...

Tags

Gram StainingBacterial MorphologyEnvironmental SamplesSoil BacteriaCrystal VioletSafranin CounterstainMicroscopy ObservationColony IsolationHeat FixingDecolorizing Agent