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HIGH SCHOOL

Chemistry

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Chemistry

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Analytical Chemistry

Ion-Exchange Chromatography for Sample Cleanup

Description

Ion-exchange chromatography is a separation method that cleans up samples by using charge. A column is packed with an ion-exchange resin, which is a charged stationary phase on a solid support. Strong cation-exchange chromatography uses a negatively charged resin to separate cations. Strong anion-exchange chromatography uses...

Transcript

Ionenuitwisselingschromatografie wordt veel gebruikt voor de scheiding en isolatie van geladen verbindingen, met name grote biomoleculen.

Dit type vloeistofchromatografie gebruikt een kolom met gepakte stationaire fase korrels, genoemd hars. De techniek scheidt analyten op basis van hun affiniteit met de geladen hars.

Er zijn twee ho...

Tags

Cation Exchange ChromatographyAnion Exchange ChromatographyProtein SeparationSalt Gradient ElutionColumn EquilibrationBuffer pH SelectionHydroxyapatite ChromatographyImmobilized Metal Affinity ChromatographyHigh Performance Liquid Chromatography

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