26.1
Microtubuli
Microtubuli zijn de dikste cytoskeletfilamenten met een diameter van 25 nm. Bij prokaryote organismen worden microtubuli vaak aangetroffen in beweging…
Microtubules are hollow cylindrical structures made up of globular proteins, alpha, and beta tubulins. With a diameter of twenty-five nanometers, they are the thickest cytoskeletal elements.
They originate from microtubule organizing centers or MTOCs and associate in linear rows to form structurally polar protofilaments with plus and minus ends.
The minus-end is the alpha-tubulin outward-facing end from where the microtubule polymerizes in the MTOC. The plus-end is the beta-tubulin outward facing end where new tubulin dimers can bind.
Microtubules act as tracks for plus-end headed kinesin and minus-end headed dynein to transport various cargoes and vesicles from one part of the cell to another.
Microtubules also bind different microtubule-associated proteins and other cytoskeletal proteins like microfilaments and intermediate filaments to maintain the cell shape and architecture.
During cell division, the microtubules help form the complex spindle apparatus. The spindle apparatus, along with the motor proteins, segregates chromosomes to the daughter cells.
View the full transcript and gain access to JoVE Core videos
Q1: What are microtubules made of and how are they structured?
Microtubules are hollow cylindrical structures composed of alpha and beta tubulin proteins that form heterodimers. With a diameter of 25 nanometers, they are the thickest cytoskeletal elements. Each microtubule contains 13 protofilaments with alternating alpha and beta tubulins arranged in a polar structure with distinct plus and minus ends.
Q2: How do kinesin and dynein use microtubules to transport cargo?
Microtubule-associated motor proteins like kinesin and dynein move along microtubules to transport cargoes and vesicles throughout the cell. Kinesin moves toward the plus end while dynein moves toward the minus end, utilizing the polar nature of microtubules as directional tracks for efficient cargo delivery and organelle positioning.
Q3: What role do GTP and its hydrolysis play in microtubule dynamics?
GTP-bound beta-tubulins at the plus end are necessary for microtubule polymerization. When GTP hydrolysis occurs on beta-tubulin, it converts to GDP and breaks lateral interactions between protofilaments, allowing tubulin monomers to dissociate. This GTP hydrolysis mechanism enables microtubules to undergo dynamic polymerization and depolymerization cycles.
Q4: Where do microtubules originate and how are they organized?
Microtubules originate from microtubule organizing centers (MTOCs) such as centrioles, where alpha-beta heterodimers assemble with help from the gamma-tubulin ring complex. The minus end originates from the MTOC, while the plus end extends outward. Microtubules associate in linear rows to form structurally polar protofilaments that radiate from the MTOC.
Q5: What functions do microtubules perform during cell division?
During cell division, microtubules help form the spindle apparatus, a complex structure essential for chromosome segregation. Working together with motor proteins, the spindle apparatus separates chromosomes and distributes them to daughter cells during mitosis, ensuring accurate genetic material transfer to each daughter cell.
Q6: How do microtubules maintain cell shape and interact with other cytoskeletal components?
Microtubules bind different microtubule-associated proteins and other cytoskeletal proteins like microfilaments and intermediate filaments to maintain cell shape and architecture. These interactions create an integrated cytoskeletal network that provides structural support and enables coordinated cellular functions and mechanical stability throughout the cell.
Q7: Why are microtubules rigid rather than flexible structures?
Microtubules are dynamic but rigid structures that cannot flex or bend when force is applied. If a deforming force is sufficiently strong, microtubules will break apart rather than bend. This rigidity makes them ideal for maintaining cell architecture and serving as stable tracks for motor protein transport.