Experimental Metastatic Melanoma Mouse Model: A Platform to Study the Metastatic Potential of Melanoma Cells in the Lungs

Samenvatting

Source: Joshua J. Timmons et al. Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs. J. Vis. Exp. (2016)

In this video, melanoma cells are intravenously injected through the tail vein in the mouse model. The melanoma cells further travel through the circulatory system, adapting to the distant microenvironment and causing metastasis in organs like lungs.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of B16-BL6 Cells

1. Remove cell culture plates from the 37-degree incubator and place them in a sterile hood. Plates should be approximately 70% confluent with B16-BL6 murine melanoma cells. A greater confluence may alter the cells' metastatic potential.
2. Add 1 mL of 0.05% Trypsin-EDTA per plate, let it sit for 1 min, and then aspirate the trypsin-media solution.
3. Add 1 mL of trypsin per plate and return plates to the incubator for 10 m....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Trypsin	Corning	MT25052CV
RPMI 1640 Medium	Corning	MT15040CM
Phase Contraast Hemacytometer	Hausser	02-671-54
Micro-Fine IV Insulin Syringes	BD	14-829-1D
Sterile Alcohol Prep Pads	Fisherbrand	22-363-750
Mouse Restrainer	Braintree Scientific	NC9999969	Restrainer choice depends on age/size of mice
Heating Pad	Harry Schein	NC0012697	Optional