Intravenous Administration of Therapeutic Nanoparticles in a Murine Glioblastoma Model

Samenvatting

Source: Kim, B. D., et al. Nanoparticle Delivery of an Oligonucleotide Payload in a Glioblastoma Multiforme Animal Model. J. Vis. Exp. (2024)

This video demonstrates the intravenous injection of therapeutic nanoparticles in a glioblastoma-bearing mouse. The method enables targeted delivery across the blood-brain barrier and supports non-invasive imaging for evaluating nanoparticle biodistribution and therapeutic efficacy in brain tumors.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. MN-anti-miR10b administration

NOTE: In this study, the injections were done once a week for 6 weeks beginning 7 days after implantation, but the same protocol can be used for various injection frequencies.

1. Weigh the mouse and calculate the volume of MN (magnetic nanoparticle)-anti-miR10b to administer to the mouse. Doses of MN-anti-miR10b are given at 20 mg Fe (iron)/kg in murine GBM (glioblastoma multiforme) models. For example, a 20 g mouse receives an 80 µL dosag....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Athymic nude "J:NU" mice	Jackson Laboratory	RRID:IMSR_JAX:007850	Immunocompromised mouse model
70% isopropoyl alcohol wipe	Cardinal	MW-APL	Topical antiseptic wipe for tumor implantation and tail vein injection
Isoflurane	Covetrus	11695067772	Anethesia
Isoflurane vaporizer	SOMNI Scientific	VS6002	Anethesia apparatus
Insulin syringe 1CC 29G X 1/2"	Becton, Dickinson	324704	Syringe for D-Luciferin injection and tail vein injection of nanoparticles