Intraperitoneal Injection of Bacterial Lipopolysaccharide in Mice to Study Systemic Immune Responses

Samenvatting

Source: Radulovic, K., et al. Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach. J. Vis. Exp. (2018)

This video demonstrates a step-by-step procedure for injecting lipopolysaccharide (LPS) into mice intraperitoneally to induce systemic inflammation.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Intraperitoneal Injection of lipopolysaccharide (LPS) to Mice
   1. Aspirate the LPS working solution in a 0.5 mL syringe with a 30 G needle in a laminar air flow.
   2. Weigh female C57Bl/6 mice (six to 10 weeks of age) and calculate the amount of LPS that will be injected: 10 µL (2 µg)/g body weight.
      NOTE: For example, if a mouse weighs 20 g, then 20 g x 10 µL = 200 µL LPS working solution needs to be injected.
   3. Handle the mouse gent....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
LPS Escherichia coli O111:B4	Invivogen, San Diego, CA, USA.	tlrl-eblps
Omnican 50 Single-use insulin syringe	B. Braun Melsungen, Melsungen, Germany	9151125