Intranasal Infection of a Mouse with a Pathogenic Influenza Virus

Samenvatting

Source: Wang, M., et al, Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice. J. Vis. Exp. (2020).

This video demonstrates intranasal infection of a mouse with a pathogenic influenza virus to establish a respiratory tract infection. The procedure outlines the administration of the virus into the nasal cavity. The virus enters host cells, replicates, and activates the immune system.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

NOTE: Virus infection of mice and isolation of organs should be performed under Animal Biosafety Level 2 (ABSL2) conditions.

1. Inoculation of PR8 influenza virus and recording of mice weight

1. Prepare 8-week-old male C57BL/6 mice for infection in the ABSL2 room.
   NOTE: This protocol is also suitable for experiments with female mice.
2. Dilution of PR8 virus: take out the virus from the -80 °C freezer and incubate on ice until it melts into liqu....