Barnes Maze: A Method For Assessing Spacial Learning and Memory in Lab Animals

Abstract

Source: Ingersoll, J. et al. Analyzing Spatial Learning and Prosocial Behavior in Mice Using the Barnes Maze and Damsel-in-Distress Paradigms. J. Vis. Exp. (2018).

This video shows how to use the Barnes Maze with a mouse. The Barnes Maze testing procedure is used by researchers to assess spatial learning and memory in laboratory animals following repeated trials.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Basic Housing of Mice

1. House the mice in plastic cages with solid bottoms and sides, and a layer of soft bedding and nesting material such as paper shredding. Use bedding that is composed of shredded corn cobs or wood shavings, and ensure that the bedding is changed regularly for sanitation.
2. Provide access to food and water ad libitum. The top of the plastic cage houses a food hopper. Dispense properly formulated mouse food pellets from the foo....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Petri Dishes	Corning	353025	Spray painted and used as covers for Barnes maze holes
Plywood (3/4 in.)	LP Building Products	22487	To construct Barnes maze
Spray Paint	Krylon	1274937	Used to paint petri dish caps black, white paint used to paint plywood
Cup Hooks (5/8 in.)	Ace Hardware	5360615	2 used on either side of ventral hole surfaces; rubberband wraps around hooks to hold cap flat
Poster Board	Creatology		Used at edges of maze as extra cues
Light Bulbs	Phillips		100W light bulb, used during the trials
Rubber bands	Sprano Brand		Size 62, used to keep caps held to plywood
Ultrasonic noisemaker	Victor mini PestChaser	M753SN	Used as aversive stimuli