Whole Mount Preparation of Mouse Intestine: Using a Simple Gut-incising Device to Prepare Whole Mount

Samenvatting

Source: Yoneda, M. et al. A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine. J. Vis. Exp. (2015)

In this video, we demonstrate whole mount preparation of the mouse intestine using a simple gut-incising device. The resulting mounts are of superior and uniform quality and can be used for histological analyses.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Device

1. Machine the main portion of the base of the device from out of a solid block of high-density acetyl resin, as described in this manuscript. At each end, create 3 mm high-elevated strips and part machine out of these 2.5 mm grooves to take the rods (see Figure 1 and Figure 2).
2. Make rods from a 2.4 mm diameter stainless steel with rounded-off ends.
NOTE:The main frame of the lid w....

Resultaten

Figure 1. Photos of the original device. The original device was constructed from several pieces of metal, prepared by hand. (A) The device assembled. (B) The top of the device has been removed to show the rods i.......

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Delrin	Ryan Plastics, Earls Barton, UK		High-density acetal resin similar material would suffice
Duralumin plate	Metal Supplies Ltd, Park Road,Dukinfield. SK16 5LP UK		Aluminium allow dating back to 1909 so alterative suppliers are available 
Finished device(s) ready for use	Contact Dr Goodlad  r.goodlad@imperial.ac.uk		Contact r.goodlad@imperial.ac.uk for supply details