Intact Ovary Harvest from Mouse: A Surgical Procedure to Isolate Complete Ovary from Murine Model

Samenvatting

Source: Mason, J. B. et al. Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice. J. Vis. Exp. (2018)

In this video, we describe a surgical procedure to harvest intact ovary from a mouse model.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Transplantation of intact ovaries to aged mice

NOTE: This protocol describes the procedure used during the transfer of ovaries from a young mouse (60 days of age) to a post reproductive female (12 months of age) of the same inbred strain. The same procedure is used for ovarian transplantation between any two female mice.

1. Weigh and anesthetize the donor animal by intraperitoneal (I.P., 27 ga needle) injection, for example u....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Dissecting microscope	Any dissecting scope with an adjustable working distance.
Warm Water Recirculator and appropriate pad for your size surgical/recovery field.	Stryker/Gaymar	TP700	Circulating water pads produce even and consistent temperature operating and recovery fields.
Surgical instruments-dissection instruments for access to the reproductive tract			Choice of specific surgical instruments is highly surgeonspecific
Suture material for ovarian bursa	eSutures	2809G: 9-0 or 2810G: 10-0 ETHILON BLACK 5" BV130-5 TAPER	10-0 suture can be difficult to locate if dropped on the surgery field.
Absorbable gelatin sponge	PFIZER	Gelfoam- PFIZER 09-0315-08	cut into small (~2mm) pieces under sterile conditions.
Staples for skin closure	Becton Dickinson	MikRon Autoclip 9mm (BD 427631)
Eye ointment.	MWI Veterinary Supply Co.		Puralube sterile opthalmic ointment
Syringes for anesthesia, analgesia and epinephrine	MWI Veterinary Supply Co	BD 305536	27g x 3/8in,Intradermal Bevel 1/2ml
Syringes for saline.	MWI Veterinary Supply Co	BD 309657.	3ml syringe, luer-lok tip, 27g needle
Clippers/blade	Andis	Super AGR+™ Cordless Detachable Blade Clipper and a CeramicEdge® #40 blade.	Any clippers with a fine blade.
Ethanol	University chemical supply		dilute to 70% for use.
Betadine solution	MWI Veterinary Supply Co.	3233
2x2 gauze pads	MWI Veterinary Supply Co.	3073	autoclaved with surgery packs
Borosilicate glass beads	Sigma-Aldrich Co	Z273619	Autoclave in sintilation vials
4-vinylcyclohexene diepoxide	Sigma-Aldrich Co.	94956