Filament Insertion-based Transient Stroke Model: A Surgical Procedure to Perform Middle Cerebral Artery Occlusion in Murine Brain

Samenvatting

Source: Llovera, G., et al. Modeling Stroke in Mice: Transient Middle Cerebral Artery Occlusion via the External Carotid Artery. J. Vis. Exp (2021).

This video describes the procedure to develop transient stroke in a murine model using silicon-coated filament-based occlusion of the middle cerebral artery. The developed model mimics human stroke, thus helping to investigate novel therapeutic strategies for treating stroke.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of the material and instruments

1. Connect the heat blanket to maintain the temperature of the operation area and the mouse body temperature during anesthesia at 37 °C.
2. Autoclave scissors and forceps, prepare 70% ethanol solution and keep available dexpanthenol eye ointment, several pieces of cotton, and 5-0 coated braided polyester suture ready for use. Prepare a 1 mL syringe with 0.9% saline solution (without needle) to keep....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
45° ramp	H&S Kunststofftechnik		height: 18 cm
5/0 threat	Pearsalls	10C103000
Anesthesia system for isoflurane	Drager
Bepanthen pomade	Bayer
C57Bl/6J mice	Charles River	000664
Clamp	FST	12500-12
Clip	FST	18055-04
Clip holder	FST	18057-14
Cottons	NOBA Verbondmitel Danz	974116
Ethanol 70%	CLN Chemikalien Laborbedorf	521005
Ethanol 96%	CLN Chemikalien Laborbedorf	522078
Ethanol 99%	CLN Chemikalien Laborbedorf	ETO-5000-99-1
Filaments	Doccol	602112PK5Re
Fine 45 angled forceps	FST	11251-35
Fine forceps	FST	11252-23
Fine Scissors	FST	14094-11
Glue	Orechseln	BSI-112
Hardener Glue	Drechseln & Mehr	BSI-151
Heating blanket	FHC DC Temperature Controller
Isoflurane	Abbot	B506
Isopentane	Fluka	59070
Laser Doppler	Perimed	PF 5010 LDPM, Periflux System 5000
Laser Doppler probe	Perimed	91-00123
Recovery chamber	Mediheat
Silicon-coated filaments	Doccol	602112PK5Re
Vannas Spring Scissors	FST	15000-00