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All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Surgical extraction of the cardiopulmonary block.
- Anesthesia:
- Use a combination of a sedative (xylazine) and a barbiturate (pentobarbital).
NOTE: Different anesthetic cocktails can be used with no effect on experimental outcomes.
- First, sedate the healthy New Zealand rabbits with a single intramuscular injection of xylazine hydrochloride (3-5 mg/kg). Ensure that the rabbits remain calm and relaxed to allow further manipulation after a f....