A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation

Samenvatting

Source: Coats, J. S. et al., Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line. J. Vis. Exp. (2017)

In this video, we demonstrate a tail-snipping procedure in a mouse model to collect peripheral blood. The collected blood is subsequently subjected to centrifugation to separate plasma, which can be used for further experiments.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Serial Blood Collection and Plasma Monitoring for Exogenous Human Cytokine in Mice

1. Blood collection via tail snip
   1. Disinfect the biosafety hood and assemble the mouse restrainer, scissors, and other tools/supplies for the procedure.
   2. Place the mouse in a restrainer and secure the tube to minimize mouse movement.
   3. Disinfect the tail and surgical scissors with isopropyl alcohol. Apply topical ane....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Animal Implantable Nano Transponder with Canula	Trovan	ID-100B(1.25)
EMLA lidocaine anesthetic cream (obtain by presciption through animal care facility)	perscription	perscription
BD ½ cc LO-DOSE U-100 Insulin Syringe 28G½	BD	#329461
BD Microtainer Tubes with K2EDTA	BD	#365974
Centrifuge	Beckman Coulter	Alegra X-15R
FisherBrand Capillary Tubes (Heparinized)	Fisher Scientific	22-260-950
Hemocytometer	Fisher	02-671-6
MACSQuant Analyzer 10	Miltenyi Biotec	130-096-343
Mouse Pie Cage	Braintree Scientific, Inc.	MPC-1
Mouse Tail Illuminator Restrainer	Braintree Scientific, Inc.	MTI STD
Pistol Grip Implanter	Trovan	IM-300(1.25)
"NSG mice" NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ, male or female, age 6 – 8 weeks	JAX Mice	# 005557