Initiating Rapid Neurite Extension and Formation Functional Neuronal Connections

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Samenvatting

Source: M. H. Magdesian et al., Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection, J. Vis. Exp. (2017)

This video demonstrates a technique for the rapid initiation and extension of a neuron and creation of new connections with a target neuron using a microfluidic device and micromanipulation. This could be used for therapies that aim to reconnect neuronal circuits after trauma or in neurodegenerative diseases.

Protocol

1. Standardization of Neuronal Cultures Using Microfluidic Devices: Device Assembly

Select a suitable microfluidic device for the desired experiment. To connect neurons within the same population, use the Neuro Devices (Figure 1), and to connect neurons in different populations, use the Co-Culture Devices (Figure 2).
Clean and prepare the desired number of sterile coverslips or glass bottom dishes. For best results on plastic surfaces, use 35 mm dishes, on glass, use 25 mm coverslips or 35 mm glass bottom dishes. Select glass thickness based on the imaging system, for instance, 0....

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Resultaten

Cell sorting microfluidic chip process; diagram and microscope images; cellular biology technique.
Figure 1: Standardization of Neuronal Cultures using Microfluidic Devices7. (a) Device assembly: when the microfluidic devices are properly assembled on a dry surface all chambers are visible. (b) Cell plating: plate the .......