Analyzing Tooth Germ and Trigeminal Ganglia Interactions Using a Microfluidic Co-culture System

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Samenvatting

Source: Pagella, P., et al. Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices. J. Vis. Exp. (2015).

This video demonstrates the utilization of a microfluidic device to study the interactions between tooth germ pulp and trigeminal ganglia. It showcases the placement of tissues inside the microfluidic device and provides a detailed visualization of trigeminal neurite growth toward the tooth germ through the device's microgrooves. Additionally, it highlights the role of tooth-derived molecular factors in promoting or inhibiting neurite extension into the tooth germ pulp, which is crucial for ensuring efficient postnatal tooth innervation.

Protocol

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

1. Preparation of Dissection Material, Culture Media, Microfluidic Devices

Autoclave micro-dissection forceps and scissors (121 °C, sterilization time: 20 min) and store them in a sterile container.
Sterilize glass coverslips (24 mm x 24 mm) by incubating them in 1 M HCl for 24 hr on an orbital shaker at 37 °C. Wash them thrice with sterile, distilled H₂O and three times with ethanol 99%. Then dry the coverslips at 37 °C or under a sterile flow hood. Finally, autoc....

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Resultaten

Microfluidic device images A and B with color-coded components for chemical reaction analysis diagram.
Figure 1. Views of mounted microfluidic co-culture device (A) from above; (B) partially lateral. The culture chambers (cc) are highlighted in red (eosin) and blue (toluidine blue); microgrooves (mg) can be seen as a w.......