Isolation of Schwann Cells from Mouse Spinal Roots

Samenvatting

Source: He, Q., et al., & Ding, F. Purification of fibroblasts and schwann cells from sensory and motor nerves in vitro. J. Vis. Exp. (2020)

This video outlines the isolation and culturing of Schwann cells from mouse spinal roots. Isolated cells are then cultured in specific media. Schwann cells are isolated via differential digestion and adherence. They are subsequently transferred to extracellular matrix-coated coverslips for attachment and proliferation.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Isolation and culture of motor and sensory nerve fibroblasts and SC (Schwann cells)

1. Use seven-day-old Sprague-Dawley (SD) rats (n=4) from the Experimental Animal Center of Nantong University of China. Place the rats in a tank containing 5% isoflurane for 2-3 minutes, allow the animals to breathe slowly and have no independent activity, and then sanitize using 75% ethanol before decapitation.
2. Use scissors to cut the back skin for about 3 cm and remove the spinal column. Open....

Resultaten

Figure 1: The schematic diagram showing the separation and purification steps of sensory and motor Fibroblasts and SCs.

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Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
D-Hank's balanced salt solution	Gibco	14170112
DMEM	Corning	10-013-CV
Dissecting microscope	Olympus	SZ2-ILST
Fetal bovine serum (FBS)	Gibco	10099-141C
Forskolin	Sigma	F6886-10MG
0.25% (w/v) trypsin	Gibco	25200-072
Culture dishes
Centrifuge tubes
Coverslips
Poly-L-Lysine
CO2 incubator