Obtaining Cerebellum Slices from an Embryonic Chick Brain

Samenvatting

Source: Hanzel, M., et al. Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors. J. Vis. Exp. (2015).

The video demonstrates the preparation of cerebellum slices. It shows a detailed procedure for isolating the cerebellum from a chick embryo. The slices are then prepared from the dissected cerebellum using a tissue chopper for further experimental analysis.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Dissection of Chicken Embryo Day 14 (e14) Cerebellum

1. Incubate brown fertilized hens' eggs at 38 °C to embryonic Day 14.
2. Using egg scissors, decapitate the chick embryo in ovo and remove the head to a Petri dish containing ice-cold Phosphate buffer saline (PBS) (Figure 1A).
3. Using standard forceps, make incisions behind each eye all the way through the tissue, removing the eyes and upper jaw. Make a second incision all....

Resultaten

Figure 1. Dissection of the cerebellum from E14 chick embryos. (A) Decapitate the chick in the egg and remove the head into a petri dish with ice-cold PBS. Remove the lower jaw and the eyes by making an incision behind the eyes and the pharynx (d.......

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
McIlwain tissue chopper	Mickle Laboratory Engineering Ltd		Cut at 300μm for best results.