Measuring Compound Muscle Action Potentials in Mouse Forelimb Muscles In Vivo

Samenvatting

Source: Pollari, E. et al., In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration. J. Vis. Exp. (2018).

This video demonstrates an in vivo technique for measuring compound muscle action potential (CMAP) in mice. It outlines the steps for setting up electrodes, stimulating forelimb neurons that innervate muscle fibers, and recording muscle responses. The technique assesses neuronal demyelination and functional neuron loss.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Animal Preparation and Anesthesia

1. Induce anesthesia in the mouse with isoflurane/oxygen inhalation. Use 4% of isoflurane for the induction of the anesthesia and 2-3% for the maintenance at 2.5 L/min flow of oxygen. Adjust the isoflurane percentage for the maintenance of the anesthesia according to the condition of the mouse, i.e., small and weak mice require less anesthetics. Confirm adequate anesthesia e.g., by applying mild pressure to the hindlim....

Resultaten

Figure 1. Positioning of the electrodes for CMAP measurements. The position of the electrodes is presented for hind- (A) and forelimbs (B). The electrodes are numbered as follows: 1: anode and 2: cathode stimulating el.......

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Resuable subdermal needle electrode, Pl/Ir	Technomed	TE/S61-434	The Needle is 13 mm (0.51") in length, 0.4 mm (27G) in diameter
Natus electrodiagnostic system	Natus Neurology	UltraPro S100	EMG device
Synergy	Natus Neurology	version 20.1.0.100	EMG software for UltraPro S100
Physitem Controller	Rothacher-Medical GmbH	TCAT-2LV	Heating pad
combi-vet Base Anesthesia System Digital Flowmeter with TEC 3 Vaporize	Rothacher & Partner	CV 30-301-D	Isoflurane Vaporizer and flowmeter
Iso-Vet 1000 mg/g	Piramal Healthcare UK Limited	AP/DRUGS/220/96	Isoflurane
SOD1-G93A mice	The Jackson Laboratory	#002726	ALS tg and non-tg control littermates, only females
PrP-hFUS-WT3 mice	The Jackson Laboratory	#017916	ALS tg and non-tg control littermates, all groups balanced for males and females
C57BL/6Jax mice	The Jackson Laboratory	#000664	Non-tg mice for axotomy, male and female
C61-PMP22 mice	Mouse line was generously donated by Prof. M. Sereda (The Max Planck Institute of Experimental Medicine, Göttingen, Germany).		CMT tg and non-tg control littermates, all groups balanced for males and female