Recording Brain Electrical Activity in Behaving Mice Using Multi-Shank Linear Silicon Probes

Samenvatting

Source: Sauer, J., et al. Recording Spatially Restricted Oscillations in the Hippocampus of Behaving Mice. J. Vis. Exp. (2018).

This video outlines a protocol for implanting a multi-shank linear silicon probe into the mouse brain to measure local field potentials. The procedure involves securing the probe to a stereotaxic unit, positioning it accurately over the target brain region, inserting it into the brain, and securing it with dental cement. After implantation, the assembly is protected with a casing. Following recovery, the setup allows for recording local field potentials, providing insights into neuronal activity.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparations

1. Design and build an appropriate insertion tool transiently carrying the silicon probe and the electrode connector during the process of implantation. See Figure 1 for an example custom built insertion tool.
2. Carefully release the silicon probe and electrode connector from its packaging using ceramic-tipped forceps.
3. Lift the connector board and securely fix it with a crocodile clamp attached to a stand.
4. Using ....

Resultaten

Figure 1: Image of the insertion tool. The silicon probe is attached to an insect pin glued to the base of a crocodile clamp. The shaft of the holder can be inserted in a stereotaxic micro manipulator for implantation.

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Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Crocodile clamp with stand	Reichelt Elektronik	HALTER ZD-10D
Silicon probe	Cambridge Neurotech	P-series 32
Stereoscope	Olympus	SZ51
Varnish-insulated copper wire	Bürklin Elektronik	89 F 232
Ground screws	Screws & More GmbH (screwsandmore.de)	DIN 84 A2 M1x2
Flux	Stannol	114018
Ceramic-tipped forceps	Fine Science Tools	11210-60
Paraffine Wax	Sigma-Aldrich	327204
Cauterizer	Fine Science Tools	18010-00
Soldering iron	Kurtz Ersa	OIC1300
Ethanol	Carl Roth	9065.1
Pasteur pipettes	Carl Roth	EA65.1
Heat sterilizer	Fine Science Tools	18000-45
Stereotaxic frame	David Kopf	Model 1900
Stereotaxic electrode holder	David Kopf	Model 1900
Isoflurane	Abbvie	B506
Oxygen concentrator	Respironix	1020007
Buprenorphine	Indivior UK Limited
Electrical shaver	Tondeo	Eco-XS
Heating pad	Thermolux	463265/-67
Surgical clamps	Fine Science Tools	18050-28
Hydrogen peroxide	Sigma-Aldrich	H1009
Sterile cotton wipes	Carl Roth	EH12.1
Drill	Proxxon	Micromot 230/E
21G injection needle	B. Braun	4657527
Phosphate buffer/phosphate buffered saline
Stereotaxic atlas	Elsevier	9.78E+12
Surgical scissors	Fine Science Tools	14094-11
Surgical forceps	Fine Science Tools	11272-40
27G injection needles	B. Braun	4657705
Vaseline
Dental cement	Sun Medical	SuperBond T&M
Carprofen	Zoetis	Rimadyl 50mg/ml
Recording amplifier	Intan Technologies	C3323
USB acquisition board	Intan Technologies	C3004
Recording cables	Intan Technologies	C3216
Electrical commutator	Doric lenses	HRJ-OE_FC_12_HARW