Analyzing Sleep Behavior in Mice through Electroencephalogram and Electromyogram Recordings

Samenvatting

Source: Oishi, Y., et  al. Polygraphic Recording Procedure for Measuring Sleep in Mice. J. Vis. Exp. (2016).

This video demonstrates a protocol for implanting an electroencephalogram (EEG) and electromyogram (EMG) electrode assembly in an anesthetized mouse. The process involves securing the mouse on a stereotaxic frame, making a midline incision in the skull, drilling holes for the electrodes, and fixing the electrode unit for signal recording. After recovery, the mouse's sleep-wake behavior is analyzed using the recorded signals.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of Electrodes and Cables for EEG/EMG Recordings

1. Prepare EEG/EMG recording electrode according to the following procedure.
   Note: The electrode is disposable and can be used only for 1 animal. Plan carefully the wiring configuration for all connectors. Place marks on the connectors for the correct orientation.
   1. Solder each pin of a 4-pin header to a 2-cm stainless steel wire. In brief, hold one end of the wire to the pin, ....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
4-pin header	Hirose	A3B-4PA-2DSA(71)
Ampicillin	Meiji Seika	N/A
Analog-to-digital converter	Contec	AD16-16U(PCIEV)
Carbide cutter	Minitor	B1055
Crimp housing	Hirose	DF11-4DS-2C
Crimp socket	Hirose	DF11-30SC
Dental cement (Toughron Rebase)	Miki Chemical Product	N/A
Epoxy adhesive	Konishi	#16351
FFC/FPC connector	Honda Tsushin Kogyo	FFC-10BMEP1(B)
Flat cable	Hitachi Cable	20528-ST LF
Instant glue (Aron Alpha A)	Toagosei	N/A
Meloxicam	Boehringer Ingelheim	N/A
Pentobarbital	Kyoritsu Seiyaku	N/A
Signal amplifier	Biotex	N/A
Sleep recording chamber	APL	N/A
SleepSign software	Kissei Comtec	N/A	for EEG/EMG recording/analysis
Slip ring	Biotex	N/A
Stainless steel screw	Yamazaki	N/A	φ1.0×2.0
Stainless steel wire	Cooner Wire	AS633