Extracellular Recording of Electrical Activity in the Drosophila Central Nervous System

In dit artikel

Samenvatting

Source: Swale, D. R., et al. Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster. J. Vis. Exp. (2018)

The video demonstrates the extracellular recording of neuronal activity from the central nervous system (CNS) of Drosophila larvae. The transected CNS is positioned in a recording chamber, and the electrical activity from a descending nerve is recorded to establish the baseline firing frequency. A test agent is then introduced, and the change in firing frequency is recorded to determine the agent's excitatory or inhibitory effect.

Protocol

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

1. Dissect and Prepare the Larval Drosophila CNS

NOTE: Methods for larval CNS dissection are clearly illustrated in Hafer and Schedl, but these previously published methods reduce the length of the descending neurons that are important for measuring spike frequency. Here, an additional method is outlined to excise the larval CNS that maintains long, intact descending neurons.

Saline preparation.
1. Prepare the dissection and recording sal....

Toegang beperkt. Log in of start een proefperiode om deze inhoud te bekijken.

Resultaten

Larval dissection, highlighting mouth hooks, ventral ganglia, cerebral lobes; dissecting scope.
Figure 1: Method for excising the CNS from third-instar maggots. (A) Intact maggot submerged in 200 µL of saline. The arrow indicates the mouth hooks that are used for separation of the body wall. (B) Two pairs of forceps are placed in .......