Selective Manipulation of Theta Oscillations in a Mouse Model

Samenvatting

Source: Bender, F. et al., Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice. J. Vis. Exp (2018)

This video demonstrates a protocol to modulate theta oscillations in a genetically engineered mouse model with light-sensitive ion channels in its GABAergic medial septum neurons. By delivering light pulses to the hippocampus, the protocol aims to synchronize neuronal activity and generate theta oscillations, which are recorded as local field potentials.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

PV-Cre knock-in male mice, 10-25 weeks old, were used. Mice were housed under standard conditions in the animal facility and kept on a 12-light/dark cycle.

1. Preparation of Tungsten Wire Arrays for LFP Recordings

1. Glue one optic fiber to the wire array so that the tip of the fiber is at the level of the shortest wire and the fiber tip is in close proximity, but not touching the tungsten wires. Keep the angle of the fiber as small as possible ....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
PV-Cre mice	The Jackson Laboratory	B6;129P2-Pvalbtm1(cre)Arbr/J
473 nm DPSS laser	Laserglow Technologies, Toronto, ON, Canada	R471005FX	LRS-0473 Series
593 nm DPSS laser	Laserglow Technologies	R591005FX	LRS-0594 Series
MC_Stimulus II	Multichannel Systems, Reutlingen, Germany	STG 4004
Tungsten wires	California Fine Wire Company, Grover Beach, CA, USA	CFW0010954	40 µm, 99.95%
Headstage	Neuralynx, Bozeman, Montana USA	HS-8	miniature headstage unity gain preamplifiers
LED	Neuralynx	HS-LED-Red-omni-10V
MATLAB	Mathworks, Natick, MA, USA
MC_Stimulus software	Multichannel, Systems
Multimode optic fiber	ThorLabs, Dachau, Germany	FG105LCA	0.22 NA, Low-OH, Ø105 µm Core, 400 - 2400 nm
Multimode fiber optic coupler	Thorlabs	FCMM50-50A-FC	1x2 MM Coupler, 50:50 Split Ratio, 50 µm GI Fibers, FC/PC