Navigational Behavior of Drosophila Larvae in Response to Optogenetic Stimulation of Olfactory Sensory Neurons

In dit artikel

Samenvatting

Source: Clark, D. A., et al. Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons. J. Vis. Exp. (2018).

This video demonstrates a study of the navigational behavior of Drosophila larvae in response to optogenetic stimulation of olfactory sensory neurons (ORNs). Transgenic Drosophila larvae expressing light-sensitive ion channels in the ORNs are placed on agarose within a behavior arena. Optogenetic stimulation with light opens the channels, causing a cation influx that triggers the transmission of the signal to the brain, and the navigation behavior is recorded.

Protocol

1. Building a Behavior Arena and Preparing Hardware to Enable Optogenetic Stimulation in the Behavior Arena

To build a light-deprived behavior arena, construct a box with a dimension of 89 x 61 x 66 cm³ (35" L x 24" W x 26" H) made of black colored plexiglass acrylic sheets (3 mm thick) (see Table of Materials). Materials to build such a box should be available at local hardware stores. Place this box on a table-top in the behavior room (Figure 1A).
Mount a monochrome universal serial bus (USB) 3.0 charge-coupled device (CCD) camera fitted with an infrared (IR) long-pass 830 nm filter ....

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Resultaten

Fluorescence imaging setup, red LED array, growth analysis; diagram with computer, LED chamber, petri dish.
Figure 1: Behavior arena and larval crawling medium. (A) Front view of the black-box behavior arena. The open door of the arena reveals a CCD camera suspended from the ceiling of the box. (B) Bottom view of a metal platform conta.......