Synaptic Modulation in Drosophila After Exposure to Prolonged Light

Samenvatting

Source: Sugie, A., et al. Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light. J. Vis. Exp. (120)

This video demonstrates the rearing of Drosophila melanogaster under specific light conditions to study the role of Bruchpilot proteins in neurotransmitter release and the resulting modulation of synaptic activity.

Protocol

1. Light Exposure Conditions

1. From the larva stage until eclosion, keep the flies in a 12 h light/12 h dark cycle (LD) at 25 °C (Figure 1A).
2. Collect the flies 0 - 6 h after eclosion and place them into a new vial containing fly food.
3. Set the vials into a transparent acrylic resin rack with three steps (a height of 41 cm, a base of 21 cm, a thickness of 4 cm, and a height of 13 cm for each step) (Figure 1B).
4. Adjust the distance between the rack and an LED panel to provide illumination with an average intensity of 1,000 lux using a digital light meter (Figure 1C).
5. Keep the flies for 1 - 3 d under one of the following conditions: constant darkness (DD), 12 h light/12 h dark cycle (LD), or constant light (LL) at 25 °C in a small incubator (Figures 1A and 1C).

Resultaten

Figure 1: Preparation of Conditions for Exposure to Defined Light Intensity. (A) Light exposure protocols after eclosion. DD, continuous darkness; LD, 12 h light/12 h dark; LL, constant exposure to light. Fly brains were dissected at the times indicated in the ...

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Vial	Hightech, Japan	MKC-20
Plug	Thermo Fisher Sciehtific, USA	AS-275
Customized transparent rack made of acrylic resin	Shin-Shin Corporation, Japan		a height of 41 cm, a base of 21 cm, a thickness of 4 cm and a height of 13 cm for each step
Cool incubator	MITSUBISHI ELECTRIC, Japan	CN-40A
LED panel	MISUMI, Japan	LEDXC170-W
Digital light meter	CEM	DT-1301
Fly pad	Tokken, Japan	TK-HA03-S