Electroencephalographic Recording of Epileptic Seizures in Epilepsy-Induced Rats

Samenvatting

Source: Soukupová, M., et al. Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats. J. Vis. Exp. (2018)

This video demonstrates electroencephalographic (EEG) recording of epileptic seizures in epilepsy-induced rats. A rat with electrodes implanted in the ventral hippocampus is connected to the EEG recording system and placed in a plexiglass chamber. The rat is allowed to move freely while the brain activity is recorded to monitor the occurrence of seizures.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Assembly of the Microdialysis Probe-electrode Device

1. Use a 3-channel two-twisted electrode (with at least a 20 mm cut length of the registering electrode and a 10 cm long grounding electrode) and couple it to a guide cannula to prepare the device. See examples of 3-channel electrodes and guide cannula for dialysis in Figure 1A-1B.
2. Remove (Figure 1C) and insert (Figure 1D) the metal guide cannula into the d....

Resultaten

Figure 1. Step-by-step preparation of the device to be implanted. (A) 3-channel electrode with 10 cm long grounding electrode in its protective sleeve on the left and guide cannula for microdialysis on the right needed to assemble the device. (B). The ba.......

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
3-channel two-twisted electrode	Invivo1, Plastic One, Roanoke, Virginia, USA	MS333/3-B/SPC	Material
guide cannula	Agn Tho's, Lindigö, Sweden	MAB 4.15.IC	Material
Resin KK2 Plastik	Elettra Sport, Lecco, Italy	KK2	Material
Super Attack gel Loctite	Henkel Italia Srl, Milano, Italy	2047420_71941	Material
Imalgene-Ketamine	Merial, Toulouse, France	221300288 (AIC)	Solution
Xylazine	Sigma, Milano, Italy	X1251	Material
Isoflurane-Vet	Merial, Toulouse, France	103120022 (AIC)	Solution
Altadol 50 mg/ ml - tramadol	Formevet, Milano, Italy	103703017 (AIC)	Solution
Gentalyn 0.1% crm - gentamycine	MSD Italia, Roma, Italy	20891077 (AIC)	Material
simplex rapid dental cement	Kemdent, Associated Dental Products Ltd, Swindon, United Kingdom	ACR811	Material
GlasIonomer CX-Plus Cement	Shofu, Kyoto, Japan	PN1167	Material
probe clip holder	Agn Tho's, Lindigö, Sweden	p/n 100 5001	Equipment
Histoacryl® Blue Topical Skin Adhesive	TissueSeal, Ann Arbor, Michigan, USA	TS1050044FP	Material
Valium 10 mg/2 ml - diazepam	Roche, Monza, Italy	019995063 (AIC)	Material
1 mL syringe with 25G needle	Vetrotecnica, Padova, Italy	11.3500.05	Material
rat flexible feeding needle 17G	Agn Tho's, Lindigö Sweden	7206	Material
Grass Technology apparatus	Grass Technologies, Natus Neurology Incorporated, Pleasanton, California, USA	M665G08	Equipment (AS40 amplifier, head box, interconnecting cables, telefactor model RPSA S40)
modular data acquisition and analysis system MP150	Biopac, Goleta, California, USA	MP150WSW	Equipment
digital video surveillance system	AverMedia Technologies, Fremont, California, USA	V4.7.0041FD	Equipment