Triggering a Closed-Loop Stimulation by Neuron Spiking Activity

Abstract

Source: Molina, L. et al. Using Neuron Spiking Activity to Trigger Closed-Loop Stimuli in Neurophysiological Experiments. J. Vis. Exp. (2019).

This video demonstrates the operation of closed-loop stimulation in a rat with a surgically implanted device to record neural activity. Neuronal signals are first recorded and analyzed. Template waveforms are then uploaded, and parameters are defined to trigger closed-loop stimulation.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Surgery

NOTE: The exact details of the surgery for closed-loop stimulation depend on the type of recording probes used and the brain areas targeted. In most cases, however, a typical surgery will consist of the following steps.

1. Bring a cage with a rat to the surgery room to be implanted with a silicone probe or electrode array to record neuronal activity.
2. Anesthetize the rodent with 2-2.5% isoflurane and fix the ....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Baytril	Bayer, Mississauga, CA	DIN 02169428	antibiotic; 50 mg/mL
Cheetah 6.4	NeuraLynx, Tucson, AZ	6.4.0.beta	Software interfaces for data acquisition
Digital Lynx 4SX	NeuraLynx, Tucson, AZ	4SX	recording equipment
Headstage transmitter	TBSI	B10-3163-GK	transmits the neural signal to the receiver
Isoflurane	Fresenius Kabi, Toronto, CA	DIN 02237518	inhalation anesthetic
Jet Denture Powder & Liqud	Lang Dental, Wheeling, US	1230	dental acrylic
Lacri-Lube	Allergan, Markham, CA	DIN 00210889	eye ointment
Lido-2	Rafter 8, Calgary	DIN 00654639	local anesthetic; 20 mg/mL
Matlab	Mathworks	R2018b	software for signal processing and triggering external events
Metacam	Boehringer, Ingelheim, DE	DIN 02240463	analgesic; 5 mg/mL
Netcom	NeuraLynx	v1	Application Programming Interface (API) that communicates with Cheetah
Silicone probe	Cambridge Neurotech	ASSY-156-DBC2	implanted device
SpikeSort 3D	NeuraLynx, Tucson, AZ	SS3D	spike waveform-to-cell classification tools
Wireless Radio Receiver	TBSI	911-1062-00	transmits the neural signal to the Digital Lynx