Handling-Induced Seizures in a Mouse Model

Samenvatting

Source: Batot, G., et al. A Model for Epilepsy of Infectious Etiology using Theiler's Murine Encephalomyelitis Virus. J. Vis. Exp. (2022).

This video demonstrates the induction of handling-induced seizures in a mouse infected with Theiler's Murine Encephalomyelitis Virus (TMEV). The infected mouse, which has hyperexcitable neurons, is exposed to external physical stimuli. This exposure causes stress, leading to abnormal electrical activity in the neurons, which results in seizures.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Virus inoculation

1. Sterilization of the injection syringe
   1. Remove the cap of the insulin syringe. Add polyethylene tubing as a collar around the needle to ensure an adequate injection depth of 2.5 mm. Submerge the syringe in ethanol for 30 min. Place the syringe under UV light for 30 min.
   2. Put the cap back onto the needle. Wrap the syringe in a sterilizing pouch and tape it closed. Label with the date of preparation.
2. Virus ....

Resultaten

Figure 1: Schematic of TMEV injection procedure. From left to right: For a right parietal cortex injection, the needle is injected slightly lateral of an imaginary line between the eye and opposite ear. The collar for depth control is indicated in yell.......

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Animal balance	Ohaus (Parsippany, NJ, U.S.A.)	STX2202	0.01 g–2200 g
BD Lo-Dose U-100 Insulin Syringes	BD (Mississauga, ON, Canada)	BD329461	Lo-Dose sterile syringes with permanent BD Micro-Fine IV needle - 1 mL
Daniel's strain of TMEV	kindly provided by Robert Fujinami (University of Utah)	-	3 x 105 plaque-forming units aliquot(s)
Disinfectant, e.g. VennoVet 1 super	Menno Chemie Vertriebsgesellschaft GmbH, Germany	-	Recommended by campus veterinarians with less than or equal to 5% alcohol
Fisherbrand medium sterile Alcohol prep pad C7	Thermo Fisher Scientific (Waltham, MA, U.S.A.)	22-363-750
Fluriso	VETone (Boise, ID, U.S.A)	502017	Isoflurane 250 mL, 2%–5%
Fume absorber	Labconco (Kansas City, MO, U.S.A.)	-	-
General Protection Disposable SMS White Lab Coats	Thermo Fisher Scientific (Waltham, MA, U.S.A.)	17-100-810A
Ice bucket	-	-	any
Mouse cage	-	-	any mouse cage holding at least 5 mice
PrecisionGlide needles	BD (Mississauga, ON, Canada)	329652	BD Slip Tip with PrecisionGlide Needle Insulin Syringes - 26 G x 3/8 - 0.45 mm x 10 mm
Self-Sealing Sterilizing Pouch	Fisher Scientific (Hampton, NY, U.S.A.)	NC9241087	12.6 x 25.5 cm
Small glass flask	-	-	any, volume 25 mL
sterile PBS	Thermo Fisher Scientific (Waltham, MA, U.S.A.)	10010056
Syringe Luer-Lok	BD (Mississauga, ON, Canada)	309628	1 mL syringe only
Ultrasonic Cleaner, Heater/Mechanical Timer	Cole-Parmer (Vernon Hills, IL, U.S.A.)	EW-08895-23	Bath sonicator - 0.5 gal, 115 V